To, Ho; Maldonado, Jaime; Tsutsumi, Nobuyuki; Gottschalk, Marcelo; Frey, Joachim; Nagai, Shinya (2024). Characterization of Actinobacillus pleuropneumoniae biovar 2 isolates reportedly reacted with the serovar 4 antiserum, and development of a multiplex PCR for O-antigen typing. Veterinary microbiology, 291(110030), p. 110030. Elsevier 10.1016/j.vetmic.2024.110030
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We have analyzed the capsule (CPS) and the lipopolysaccharide O-Antigen (O-Ag) biosynthesis loci of twelve Spanish field isolates of Actinobacillus pleuropneumoniae biovar 2, eleven of them previously typed serologically as serovar 4 and one non-typable (NT) (Maldonado et al., 2009, 2011). These isolates have the common core genes of the type I CPS locus, sharing >98% identity with those of serovar 2. However, the former possesses the O-Ag locus as serovar 4, and the latter possesses the O-Ag locus as serovar 7. The main difference found between the CPS loci of the 11 isolates and that of serovar 2 reference strain S1536 are two deletions, one of an 8 bp sequence upstream of the coding sequence and one of 111 bp sequence at the 5' end of the cps2G gene. The deletion mutations mentioned lead to a defect in the production of CPS in these isolates, which contributed to their previous mis-identification. In order to complement the serotyping of A. pleuropneumoniae in diagnostics and epidemiology, we have developed a multiplex PCR for the comprehensive O-Ag typing of all A. pleuropneumoniae isolates.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
05 Veterinary Medicine > Other Institutions > Office of the Dean 05 Veterinary Medicine > Other Institutions > Emeriti, Vetsuisse Faculty |
UniBE Contributor: |
Frey, Joachim |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
0378-1135 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
04 Mar 2024 16:23 |
Last Modified: |
13 Mar 2024 00:16 |
Publisher DOI: |
10.1016/j.vetmic.2024.110030 |
PubMed ID: |
38428226 |
Uncontrolled Keywords: |
Actinobacillus pleuropneumoniae MPCR O-antigen Serovar K2:O4 Swine |
BORIS DOI: |
10.48350/193727 |
URI: |
https://boris.unibe.ch/id/eprint/193727 |
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