Tomasiak, Alicja; Berg, Lea Sophie; Sala, Katarzyna; Braszewska, Agnieszka (2024). Quantitative Analysis of Epigenetic Modifications in Fagopyrum Nuclei with Confocal Microscope, ImageJ, and R Studio. Methods in molecular biology, 2791, pp. 23-33. Springer 10.1007/978-1-0716-3794-4_3
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Epigenetic programming plays a vital role in regulating pluripotency genes, which become activated or inactivated during the processes of dedifferentiation and differentiation during an organism's development. The analysis of epigenetic modifications has become possible through the technique of immunostaining, where specific antibodies allow the identification of a single target protein. This chapter describes a detailed protocol for the analysis of the epigenetic modifications with the use of confocal microscopy, subsequent image, and statistical analysis on the example of Fagopyrum calli with the use of nine antibodies raised against histone H3 and H4 methylation and acetylation on several lysines as well as DNA methylation.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) |
UniBE Contributor: |
Berg, Lea Sophie |
Subjects: |
500 Science > 580 Plants (Botany) |
ISSN: |
1940-6029 |
Publisher: |
Springer |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
28 Mar 2024 14:14 |
Last Modified: |
29 Mar 2024 14:37 |
Publisher DOI: |
10.1007/978-1-0716-3794-4_3 |
PubMed ID: |
38532089 |
Uncontrolled Keywords: |
Confocal microscopy Epigenetics R Studio |
BORIS DOI: |
10.48350/194956 |
URI: |
https://boris.unibe.ch/id/eprint/194956 |
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