Pilotto, Federica; Diab, Rim; Al Qassab, Zahraa; Saxena, Smita (2024). Generation and enrichment of cerebellar GABAergic interneurons from human induced pluripotent stem cells and intracellular calcium measurements. STAR protocols, 5(2), p. 102936. Elsevier 10.1016/j.xpro.2024.102936
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GABAergic interneurons are inhibitory neurons of the CNS, playing a fundamental role in neural circuitry and activity. Here, we provide a robust protocol for the successful enrichment of human cerebellar GABAergic interneurons from human induced pluripotent stem cells (iPSCs) and measuring intracellular calcium transients. We describe in detail steps for culturing iPSCs; generating embryoid bodies; and differentiating and enriching for cerebellar GABAergic neurons (cGNs), with precise steps for their molecular characterization. We then detail the procedure for adeno-associated virus-mediated transduction of cGNs with genetically encoded calcium indicators, followed by intracellular calcium imaging and analyses. For complete details on the use and execution of this protocol, please refer to Pilotto et al.1.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DCR Unit Sahli Building > Forschungsgruppe Neurologie 04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Neurology 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) |
UniBE Contributor: |
Diab, Rim, Al Qassab, Zahraa Ahmed Mohammed, Saxena, Smita |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2666-1667 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
13 May 2024 12:57 |
Last Modified: |
26 Jun 2024 00:14 |
Publisher DOI: |
10.1016/j.xpro.2024.102936 |
PubMed ID: |
38735042 |
Uncontrolled Keywords: |
Cell Biology Neuroscience Stem Cells |
BORIS DOI: |
10.48350/196714 |
URI: |
https://boris.unibe.ch/id/eprint/196714 |
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