Christensen, Henrik; Kuhnert, Peter; Foster, Geoff; Bisgaard, Magne (2024). Mannheimia indoligenes sp. nov., proposed for clade V organisms of Mannheimia. International journal of systematic and evolutionary microbiology, 74(5) Microbiology Society 10.1099/ijsem.0.006370
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A set of 25 strains belonging to clade V of Mannheimia mainly isolated from cattle was investigated and is proposed to represent Mannheimia indoligenes sp. nov. The species can be separated from the other validly published species of the genus by pheno- and genotype. Only indole separates M. indoligenes and Mannheimia varigena while two to seven characters separate M. indoligenes from other species of Mannheimia. Thirteen strains belonging to biogroups 6, 7, 8C, 9, 10, 12 and UG5 formed a monophyletic group based on 16S rRNA gene sequence comparisons with 98-100 % similarity. Eight of these strains were further included in the whole genome comparison. Digital DNA-DNA hybridization showed that the similarities between the suggested type strain M14.4T and the other strains of M. indoligenes were 62.9 % or higher. The average nucleotide identity was 95.5 % or higher between M14.4T and the other strains of the species. The rpoB gene sequence similarity was 95-100 % within M. indoligenes. MALDI-TOF allowed a clear separation from other Mannheimia species further supporting classification as a novel species and making it the diagnostic identification tool of choice for M. indoligenes. The type strain is M14.4T (=CCUG 77347T=DSM 116804T) isolated from a cattle tongue in Scotland.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology |
UniBE Contributor: |
Kuhnert, Peter |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
1466-5034 |
Publisher: |
Microbiology Society |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
14 May 2024 13:39 |
Last Modified: |
14 May 2024 14:12 |
Publisher DOI: |
10.1099/ijsem.0.006370 |
PubMed ID: |
38739681 |
Uncontrolled Keywords: |
MALDI-TOF MEE biogroup cAMP ruminant |
BORIS DOI: |
10.48350/196744 |
URI: |
https://boris.unibe.ch/id/eprint/196744 |
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