Smith, Harry A; Templeman, Iain; Davis, Max; Slater, Tommy; Clayton, David J; Varley, Ian; James, Lewis J; Middleton, Benita; Johnston, Jonathan D; Karagounis, Leonidas G; Tsintzas, Kostas; Thompson, Dylan; Gonzalez, Javier T; Walhin, Jean-Philippe; Betts, James A (2024). Characterising 24-h skeletal muscle gene expression alongside metabolic & endocrine responses under diurnal conditions. (In Press). Journal of clinical endocrinology and metabolism Oxford University Press 10.1210/clinem/dgae350
|
Text
dgae350.pdf - Accepted Version Available under License Creative Commons: Attribution (CC-BY). Download (1MB) | Preview |
CONTEXT
Skeletal muscle plays a central role in the storage, synthesis, and breakdown of nutrients, yet little research has explored temporal responses of this human tissue, especially with concurrent measures of systemic biomarkers of metabolism.
OBJECTIVE
To characterise temporal profiles in skeletal muscle expression of genes involved in carbohydrate metabolism, lipid metabolism, circadian clocks, and autophagy and descriptively relate them to systemic metabolites and hormones during a controlled laboratory protocol.
METHODS
Ten healthy adults (9M/1F, mean ± SD: age: 30 ± 10 y; BMI: 24.1 ± 2.7 kg·m-2) rested in the laboratory for 37 hours with all data collected during the final 24 hours of this period (i.e., 0800-0800 h). Participants ingested hourly isocaloric liquid meal replacements alongside appetite assessments during waking before a sleep opportunity from 2200-0700 h. Blood samples were collected hourly for endocrine and metabolite analyses, with muscle biopsies occurring every 4 h from 1200 h to 0800 h the following day to quantify gene expression.
RESULTS
Plasma insulin displayed diurnal rhythmicity peaking at 1804 h. Expression of skeletal muscle genes involved in carbohydrate metabolism (Name - Acrophase; GLUT4 - 1440 h; PPARGC1A -1613 h; HK2 - 1824 h) and lipid metabolism (FABP3 - 1237 h; PDK4 - 0530 h; CPT1B - 1258 h) displayed 24 h rhythmicity that reflected the temporal rhythm of insulin. Equally, circulating glucose (0019 h), NEFA (0456 h), glycerol (0432 h), triglyceride (2314 h), urea (0046 h), CTX (0507 h) and cortisol concentrations (2250 h) also all displayed diurnal rhythmicity.
CONCLUSION
Diurnal rhythms were present in human skeletal muscle gene expression as well systemic metabolites and hormones under controlled diurnal conditions. The temporal patterns of genes relating to carbohydrate and lipid metabolism alongside circulating insulin are consistent with diurnal rhythms being driven in part by the diurnal influence of cyclic feeding and fasting.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Social and Preventive Medicine (ISPM) |
UniBE Contributor: |
Karagounis, Leonidas |
Subjects: |
600 Technology > 610 Medicine & health 300 Social sciences, sociology & anthropology > 360 Social problems & social services |
ISSN: |
0021-972X |
Publisher: |
Oxford University Press |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
24 May 2024 09:08 |
Last Modified: |
12 Jun 2024 12:55 |
Publisher DOI: |
10.1210/clinem/dgae350 |
PubMed ID: |
38779872 |
Uncontrolled Keywords: |
Circadian rhythms Diurnal Gene expression Glucose Lipids Skeletal muscle |
BORIS DOI: |
10.48350/197040 |
URI: |
https://boris.unibe.ch/id/eprint/197040 |
Actions (login required)
 |
Edit item |