Zakirova, Natalia F; Khomich, Olga A; Smirnova, Olga A; Molle, Jennifer; Duponchel, Sarah; Yanvarev, Dmitry V; Valuev-Elliston, Vladimir T; Monnier, Lea; Grigorov, Boyan; Ivanova, Olga N; Karpenko, Inna L; Golikov, Mikhail V; Bovet, Cedric; Rindlisbacher, Barbara; Khomutov, Alex R; Kochetkov, Sergey N; Bartosch, Birke; Ivanov, Alexander V (2024). Hepatitis C Virus Dysregulates Polyamine and Proline Metabolism and Perturbs the Urea Cycle. Cells, 13(12) MDPI 10.3390/cells13121036
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Hepatitis C virus (HCV) is an oncogenic virus that causes chronic liver disease in more than 80% of patients. During the last decade, efficient direct-acting antivirals were introduced into clinical practice. However, clearance of the virus does not reduce the risk of end-stage liver diseases to the level observed in patients who have never been infected. So, investigation of HCV pathogenesis is still warranted. Virus-induced changes in cell metabolism contribute to the development of HCV-associated liver pathologies. Here, we studied the impact of the virus on the metabolism of polyamines and proline as well as on the urea cycle, which plays a crucial role in liver function. It was found that HCV strongly suppresses the expression of arginase, a key enzyme of the urea cycle, leading to the accumulation of arginine, and up-regulates proline oxidase with a concomitant decrease in proline concentrations. The addition of exogenous proline moderately suppressed viral replication. HCV up-regulated transcription but suppressed protein levels of polyamine-metabolizing enzymes. This resulted in a decrease in polyamine content in infected cells. Finally, compounds targeting polyamine metabolism demonstrated pronounced antiviral activity, pointing to spermine and spermidine as compounds affecting HCV replication. These data expand our understanding of HCV's imprint on cell metabolism.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Institute of Clinical Chemistry |
UniBE Contributor: |
Bovet, Cédric |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2073-4409 |
Publisher: |
MDPI |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
27 Jun 2024 10:30 |
Last Modified: |
27 Jun 2024 10:38 |
Publisher DOI: |
10.3390/cells13121036 |
PubMed ID: |
38920664 |
Uncontrolled Keywords: |
antiviral agents hepatitis C virus polyamines proline metabolism urea cycle |
BORIS DOI: |
10.48350/198148 |
URI: |
https://boris.unibe.ch/id/eprint/198148 |
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