Jacobs, Karl; Docter, Daniel; de Smit, Lotte; Korfage, Hans A M; Visser, Sophie C; Lobbezoo, Frank; Hlushchuk, Ruslan; de Bakker, Bernadette S (2024). High resolution imaging of human development: shedding light on contrast agents. (In Press). Neuroradiology Springer-Verlag 10.1007/s00234-024-03413-z
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BACKGROUND
Visualizing (micro)vascular structures remains challenging for researchers and clinicians due to limitations in traditional radiological imaging methods. Exploring the role of vascular development in craniofacial malformations in experimental settings can enhance understanding of these processes, with the effectiveness of high-resolution imaging techniques being crucial for successful research in this field. Micro-CT imaging offers 3D microstructural insights, but requires contrast-enhancing staining agents (CESAs) for visualizing (micro)-vascular tissues, known as contrast-enhanced micro-CT (CECT). As effective contrast agents are crucial for optimal visualization, this review focuses on comparative studies investigating such agents for micro-vascular tissue imaging using micro-CT. Furthermore, we demonstrate the utilization of B-Lugol solution as a promising contrast agent for acquiring high-quality micro-CT images of (micro)vascular structures in human embryonic samples.
METHOD
This scoping review followed Preferred Reporting Items for Systematic Reviews and Meta-analysis Protocols. PubMed database provided relevant articles, screened initially by title and abstract. Inclusion and exclusion criteria defined outcomes of interest.
RESULTS
From an initial search, 273 records were identified, narrowed down to 9 articles after applying our criteria. Additionally, two articles were added through citation searching. This, a total of 11 articles were incorporated in this study.
CONCLUSION
This micro-CT contrast agent review underscores the need for tailored choices based on research goals. Both Barium sulfate and Iodine-based agents showing excellent results, providing high resolution (micro) vascular content, especially in ex-vivo specimens. However, careful consideration of protocols and tissue characteristics remains imperative for optimizing the effectiveness of micro-CT imaging for the study of cranio-facial vascular development.
Item Type: |
Journal Article (Review Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy 04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Topographical and Clinical Anatomy |
UniBE Contributor: |
Hlushchuk, Ruslan |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health |
ISSN: |
0028-3940 |
Publisher: |
Springer-Verlag |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
15 Jul 2024 14:36 |
Last Modified: |
17 Jul 2024 10:19 |
Publisher DOI: |
10.1007/s00234-024-03413-z |
PubMed ID: |
38995394 |
Uncontrolled Keywords: |
Contrast agents High-resolution imaging techniques Micro-CT Microvascular tissue imaging Vascular development |
BORIS DOI: |
10.48350/198981 |
URI: |
https://boris.unibe.ch/id/eprint/198981 |
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