Human melanocytes can be isolated, propagated and expanded from plucked anagen hair follicles

Dieckmann, Christina; Milkova, Linda; Hunziker, Thomas; Emmendörffer, Andreas; Simon, Jan C (2010). Human melanocytes can be isolated, propagated and expanded from plucked anagen hair follicles. Experimental dermatology, 19(6), pp. 543-5. Oxford: Blackwell 10.1111/j.1600-0625.2009.01019.x

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Herein, we report a technically simple method for isolation and culture of human follicular melanocytes based on explant cultures of epilated hair follicles. This technique does not require any surgical intervention and allows the isolation and cultivation of follicular melanocytes from a comparatively small amount of raw material. Generally, 30-60 human anagen hair follicles have been plucked from the scalp of healthy donors and cultivated under low oxygen pressure (5%). After a short period of time cells of various types were growing out from the outer root sheath (ORS) of the hair follicles. Under the selected culture conditions, most of the cells other than melanocytes have been eliminated and a nearly 100% pure population of melanocytes has been achieved, as confirmed by immunohistochemical analyses for melanocyte-specific markers, for example, Tyrosinase-1, S-100 and premelanosomal antigens. These melanocytes derived from the ORS were proliferating for up to 2 months.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Dermatology

UniBE Contributor:

Hunziker, Thomas

ISSN:

0906-6705

Publisher:

Blackwell

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:07

Last Modified:

17 Mar 2015 19:07

Publisher DOI:

10.1111/j.1600-0625.2009.01019.x

PubMed ID:

20374294

Web of Science ID:

000277788000011

URI:

https://boris.unibe.ch/id/eprint/199 (FactScience: 196882)

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