Marq, Jean-Baptiste; Gosetto, Margaux; Altenried, Aline; Vadas, Oscar; Maco, Bohumil; Dos Santos Pacheco, Nicolas; Tosetti, Nicolò; Soldati-Favre, Dominique; Lentini, Gaëlle (2024). Cytokinetic abscission in Toxoplasma gondii is governed by protein phosphatase 2A and the daughter cell scaffold complex. The EMBO journal, 43(17), pp. 3752-3786. EMBO Press 10.1038/s44318-024-00171-9
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marq-et-al-2024-cytokinetic-abscission-in-toxoplasma-gondii-is-governed-by-protein-phosphatase-2a-and-the-daughter-cell.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (15MB) | Preview |
Cytokinetic abscission marks the final stage of cell division, during which the daughter cells physically separate through the generation of new barriers, such as the plasma membrane or cell wall. While the contractile ring plays a central role during cytokinesis in bacteria, fungi and animal cells, the process diverges in Apicomplexa. In Toxoplasma gondii, two daughter cells are formed within the mother cell by endodyogeny. The mechanism by which the progeny cells acquire their plasma membrane during the disassembly of the mother cell, allowing daughter cells to emerge, remains unknown. Here we identify and characterize five T. gondii proteins, including three protein phosphatase 2A subunits, which exhibit a distinct and dynamic localization pattern during parasite division. Individual downregulation of these proteins prevents the accumulation of plasma membrane at the division plane, preventing the completion of cellular abscission. Remarkably, the absence of cytokinetic abscission does not hinder the completion of subsequent division cycles. The resulting progeny are able to egress from the infected cells but fail to glide and invade, except in cases of conjoined twin parasites.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Cell Biology |
UniBE Contributor: |
Lentini, Gaëlle Audrey |
Subjects: |
500 Science > 570 Life sciences; biology |
ISSN: |
1460-2075 |
Publisher: |
EMBO Press |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
16 Jul 2024 13:47 |
Last Modified: |
07 Sep 2024 00:14 |
Publisher DOI: |
10.1038/s44318-024-00171-9 |
PubMed ID: |
39009675 |
Uncontrolled Keywords: |
Abscission Cytokinesis Cytoskeleton Division Plane Phosphatase |
BORIS DOI: |
10.48350/199029 |
URI: |
https://boris.unibe.ch/id/eprint/199029 |
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