Immler, Martin; Wolfram, Michael; Oevermann, Anna; Walter, Ingrid; Wolfesberger, Birgitt; Tichy, Alexander; Gradner, Gabriele (2024). Expression of somatostatin receptors in canine and feline meningioma. Veterinary medicine and science, 10(4), e1537. Wiley 10.1002/vms3.1537
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Veterinary_Medicine___Sci_-_2024_-_Immler_-_Expression_of_somatostatin_receptors_in_canine_and_feline_meningioma.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (1MB) | Preview |
OBJECTIVES
The standard treatment for canine and feline meningiomas includes radiotherapy, surgical excision or combined therapy. However, new therapeutic approaches are required due to the possible recurrence or progression of meningiomas despite initial therapy. Adjunctive therapy with synthetic long-acting somatostatin (SST) analogues has been described in humans with SST-expressing tumours. The expression of SST receptors (SSTRs) by feline meningiomas is currently unknown, and there are little data about canine meningiomas. We hypothesized that SSTR is expressed by canine and feline meningiomas (S1).
METHODS
Seven canines and 11 felines with histologically confirmed meningiomas underwent STTR screening. RNA expressions of SSTR1, SSTR2, SSTR3 and SSTR5 (canine) and SSTR1-SSTR 5 (feline) in fresh frozen and formalin-fixed and paraffin-embedded (FFPE) samples were investigated using real-time (RT)-qPCR. The expression of SSTR1 and SSTR2 in FFPE samples was evaluated using immunohistochemistry (IHC). The specificity of applied antibodies for canine and feline species was confirmed by western blotting.
RESULTS
In canine meningiomas (n = 7), RNA expression of SSTR1, SSTR2 and SSTR5 was detected in all samples; SSTR3 RNA expression was detected in only 33% of samples. In feline meningiomas (n = 12), RNA expression of SSTR1, SSTR4, SSTR5 and SSTR2 was detected in 91%, 46%, 46% and 36% of samples, respectively; SSTR3 was not expressed. Overall, the detection rate was lower in FFPE samples. IHC revealed the expression of SSTR1 and SSTR2 in all samples from both species. However, it is important to exercise caution when interpreting IHC results due to the presence of diffuse background staining.
CONCLUSIONS
SSTRs are widely expressed in canine and feline meningiomas, thereby encouraging further studies investigating SSTR expression to conduct trials about the effect of adjunctive therapy with long-acting SST-analogues.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research 05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) |
UniBE Contributor: |
Oevermann, Anna |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
2053-1095 |
Publisher: |
Wiley |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
17 Jul 2024 07:43 |
Last Modified: |
17 Jul 2024 07:53 |
Publisher DOI: |
10.1002/vms3.1537 |
PubMed ID: |
39011594 |
Uncontrolled Keywords: |
RT‐qPCR cat dog immunohistochemistry meningioma somatostatin receptor |
BORIS DOI: |
10.48350/199037 |
URI: |
https://boris.unibe.ch/id/eprint/199037 |
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