Pannek, Jürgen; Mahler, Jasmin; Kurmann, Carmen; Widmer, Alexandra; Krebs, Jörg; Wöllner, Jens (2024). An in-vitro model for bacteria-related catheter encrustations. World journal of urology, 42(1), p. 469. Springer 10.1007/s00345-024-05191-y
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PURPOSE
About 50% of individuals with long-term indwelling catheters are affected by catheter encrustations and bladder stone formation. Therefore, prophylaxis of catheter encrustations is important. Currently, however, neither an established prophylaxis nor a standardized in-vitro model to test different measures exist. We have therefore developed and qualitatively evaluated an in-vitro model of catheter encrustation.
METHODS
Size 14 French suprapubic catheters were incubated under sterile conditions at 37 degrees Celsius in five different media: (1) sterile artificial urine (n = 16), (2) artificial urine with E. coli (n = 8), (3) with Pseudomonas aeruginosa (n = 8), (4) with Proteus mirabilis (n = 8), and (5) with a mix of these three strains (n = 8). Catheter balloons were inflated either a glycerine or a bactericidal solution. After 6 weeks, the catheters were removed from the solution, dried, and weighed, and a photometric determination of the retrieved encrustations was performed.
RESULTS
Most frequently and pronounced encrustations were detected in the Pseudomonas group. The median weight of these encrustations (50% struvite and brushite) was 84.4 mg (47.7 mg / 127.3 mg). Even on catheters stored in sterile urine, encrustations (69.2% struvite) were found. Bacterial growth was not affected by the medium used for catheter blockage.
CONCLUSION
Although in-vitro models appear to be limited because they lack "the human factor", they are valuable for systematically assessing physico-chemical factors affecting encrustations. Therefore, our model, being reliable and cost-effective, may foster further research despite its limitations.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Urology |
UniBE Contributor: |
Pannek, Jürgen |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1433-8726 |
Publisher: |
Springer |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
08 Aug 2024 08:52 |
Last Modified: |
08 Aug 2024 09:00 |
Publisher DOI: |
10.1007/s00345-024-05191-y |
PubMed ID: |
39110241 |
Uncontrolled Keywords: |
Artificial urine Bacteriuria Encrustation Indwelling catheter |
BORIS DOI: |
10.48350/199574 |
URI: |
https://boris.unibe.ch/id/eprint/199574 |