Purification of Epizootic Hemorrhagic Disease Virus (and Other Orbiviruses) Particles from Infected Mammalian or Insect Cells.

Guimerà Busquets, Marc; Darpel, Karin E. (2024). Purification of Epizootic Hemorrhagic Disease Virus (and Other Orbiviruses) Particles from Infected Mammalian or Insect Cells. Methods in molecular biology, 2838, pp. 77-89. Springer 10.1007/978-1-0716-4035-7_4

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Epizootic hemorrhagic disease virus (EHDV), like other orbiviruses, infects and replicates in mammalian and insect vector cells. Within its ruminant hosts EHDV, like bluetongue virus (BTV), it has mainly been associated with infection of endothelial cells of capillaries as well as leukocyte subsets. Furthermore, EHDV infects and replicates within its biological vector, Culicoides biting midges and Culicoides-derived cells. A wide range of common laboratory cell lines such as BHK, BSR, and Vero cells are susceptible to infection with certain EHDV strains. Cell culture supernatants of infected cells are commonly used for both in vivo and in vitro infection studies. For specific virological or immunological studies, using highly purified virus particles, however, might be beneficial or even required. Here we describe a purification method for EHDV particles, which had been originally developed for certain strains of BTV.

Item Type:	Journal Article (Further Contribution)
Division/Institute:	05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
UniBE Contributor:	Darpel, Karin
Subjects:	600 Technology > 630 Agriculture
ISSN:	1940-6029
Publisher:	Springer
Language:	English
Submitter:	Pubmed Import
Date Deposited:	12 Aug 2024 13:53
Last Modified:	12 Aug 2024 13:53
Publisher DOI:	10.1007/978-1-0716-4035-7_4
PubMed ID:	39126624
Uncontrolled Keywords:	Gradient centrifugation purification Virus particle types Virus-host-vector interaction
URI:	https://boris.unibe.ch/id/eprint/199618