Torabi Rahvar, Parisa; Abdekhodaie, Mohammad J; Jooybar, Elaheh; Gantenbein, Benjamin (2024). An enzymatically crosslinked collagen type II/hyaluronic acid hybrid hydrogel: A biomimetic cell delivery system for cartilage tissue engineering. (In Press). International journal of biological macromolecules, 279(Pt 1), p. 134614. Elsevier 10.1016/j.ijbiomac.2024.134614
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This study presents new injectable hydrogels based on hyaluronic acid and collagen type II that mimic the polysaccharide-protein structure of natural cartilage. After collagen isolation from chicken sternal cartilage, tyramine-grafted hyaluronic acid and collagen type II (HA-Tyr and COL-II-Tyr) were synthesized. Hybrid hydrogels were prepared with different ratios of HA-Tyr/COL-II-Tyr using horseradish peroxidase and noncytotoxic concentrations of hydrogen peroxide to encapsulate human bone marrow-derived mesenchymal stromal cells (hBM-MSCs). The findings showed that a higher HA-Tyr content resulted in a higher storage modulus and a lower hydrogel shrinkage, resulting in hydrogel swelling. Incorporating COL-II-Tyr into HA-Tyr hydrogels induced a more favorable microenvironment for hBM-MSCs chondrogenic differentiation. Compared to HA-Tyr alone, the hybrid HA-Tyr/COL-II-Tyr hydrogel promoted enhanced chondrocyte adhesion, spreading, proliferation, and upregulation of cartilage-related gene expression. These results highlight the promising potential of injectable HA-Tyr/COL-II-Tyr hybrid hydrogels to deliver cells for cartilage regeneration.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Orthopaedic, Plastic and Hand Surgery (DOPH) > Clinic of Orthopaedic Surgery 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) |
UniBE Contributor: |
Torabi Rahvar, Parisa, Gantenbein, Benjamin |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1879-0003 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
12 Aug 2024 11:25 |
Last Modified: |
01 Sep 2024 00:20 |
Publisher DOI: |
10.1016/j.ijbiomac.2024.134614 |
PubMed ID: |
39127277 |
Uncontrolled Keywords: |
Biomimetic materials Cell encapsulation Collagen type II Enzymatic crosslinking Tissue engineering |
BORIS DOI: |
10.48350/199632 |
URI: |
https://boris.unibe.ch/id/eprint/199632 |
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