Myocardial injection of skeletal myoblasts impairs contractility of host cardiomyocytes

Giraud, Marie-Noëlle; Liechti, Emanuel F.; Tchantchaleishvili, Vakhtang; Siepe, Matthias; Cook, Stéphane; Carrel, Thierry P; Tevaearai, Hendrik T (2010). Myocardial injection of skeletal myoblasts impairs contractility of host cardiomyocytes. International journal of cardiology, 138(2), pp. 131-137. Amsterdam: Elsevier 10.1016/j.ijcard.2008.08.034

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BACKGROUND: Mechanisms underlying improvement of myocardial contractile function after cell therapy as well as arrhythmic side effect remain poorly understood. We hypothesised that cell therapy might affect the mechanical properties of isolated host cardiomyocytes. METHODS: Two weeks after myocardial infarction (MI), rats were treated by intramyocardial myoblast injection (SkM, n=8), intramyocardial vehicle injection (Medium, n=6), or sham operation (Sham, n=7). Cardiac function was assessed by echocardiography. Cardiomyocytes were isolated in a modified Langendorff perfusion system, their contraction was measured by video-based inter-sarcomeric analysis. Data were compared with a control-group without myocardial infarction (Control, n=5). RESULTS: Three weeks post-treatment, ejection fraction (EF) further deteriorated in vehicle-injected and non-injected rats (respectively 40.7+/-11.4% to 33+/-5.5% and 41.8+/-8% to 33.5+/-8.3%), but was stabilised in SkM group (35.9+/-6% to 36.4+/-9.7%). Significant cell hypertrophy induced by MI was maintained after cell therapy. Single cell contraction (dL/dt(max)) decreased in SkM and vehicle groups compared to non-injected group as well as cell shortening and relaxation (dL/dt(min)) in vehicle group. A significantly increased predisposition for alternation of strong and weak contractions was observed in isolated cardiomyocytes of the SkM group. CONCLUSION: Our study provides the first evidence that injection of materials into the myocardium alters host cardiomyocytes contractile function independently of the global beneficial effect of the heart function. These findings may be important in understanding possible adverse effects.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Cardiology
04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Cardiovascular Surgery

UniBE Contributor:

Giraud, Marie-Noelle; Cook, Stéphane; Carrel, Thierry and Tevaearai, Hendrik

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0167-5273

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:07

Last Modified:

20 Jan 2015 08:29

Publisher DOI:

10.1016/j.ijcard.2008.08.034

PubMed ID:

18809218

Web of Science ID:

000273613300005

BORIS DOI:

10.7892/boris.2

URI:

https://boris.unibe.ch/id/eprint/2 (FactScience: 119557)

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