Comparison of bacterial plaque samples from titanium implant and tooth surfaces by different methods

Gerber, Jeanne; Wenaweser, Doris; Heitz-Mayfield, Lisa; Lang, Niklaus Peter; Persson, Gösta Rutger (2006). Comparison of bacterial plaque samples from titanium implant and tooth surfaces by different methods. Clinical oral implants research, 17(1), pp. 1-7. Oxford: Wiley-Blackwell 10.1111/j.1600-0501.2005.01197.x

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Studies have shown similarities in the microflora between titanium implants or tooth sites when samples are taken by gingival crevicular fluid (GCF) sampling methods. The purpose of the present study was to study the microflora from curette and GCF samples using the checkerboard DNA-DNA hybridization method to assess the microflora of patients who had at least one oral osseo-integrated implant and who were otherwise dentate. Plaque samples were taken from tooth/implant surfaces and from sulcular gingival surfaces with curettes, and from gingival fluid using filter papers. A total of 28 subjects (11 females) were enrolled in the study. The mean age of the subjects was 64.1 years (SD+/-4.7). On average, the implants studied had been in function for 3.7 years (SD+/-2.9). The proportion of Streptococcus oralis (P<0.02) and Fusobacterium periodonticum (P<0.02) was significantly higher at tooth sites (curette samples). The GCF samples yielded higher proportions for 28/40 species studies (P-values varying between 0.05 and 0.001). The proportions of Tannerella forsythia (T. forsythensis), and Treponema denticola were both higher in GCF samples (P<0.02 and P<0.05, respectively) than in curette samples (implant sites). The microbial composition in gingival fluid from samples taken at implant sites differed partly from that of curette samples taken from implant surfaces or from sulcular soft tissues, providing higher counts for most bacteria studied at implant surfaces, but with the exception of Porphyromonas gingivalis. A combination of GCF and curette sampling methods might be the most representative sample method.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology

UniBE Contributor:

Gerber, Jeanne; Heitz-Mayfield, Lisa; Lang, Niklaus Peter and Persson, Gösta Rutger

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0905-7161

Publisher:

Wiley-Blackwell

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

04 Oct 2013 14:48

Last Modified:

25 Jan 2017 12:17

Publisher DOI:

10.1111/j.1600-0501.2005.01197.x

PubMed ID:

16441779

Web of Science ID:

000234854400001

BORIS DOI:

10.7892/boris.20033

URI:

https://boris.unibe.ch/id/eprint/20033 (FactScience: 3114)

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