Design of custom-shaped vascularized tissues using microtissue spheroids as minimal building units

Kelm, Jens M; Djonov, Valentin; Ittner, Lars M; Fluri, David; Born, Walter; Hoerstrup, Simon P; Fussenegger, Martin (2006). Design of custom-shaped vascularized tissues using microtissue spheroids as minimal building units. Tissue engineering, 12(8), pp. 2151-60. Larchmont, N.Y.: Mary Ann Liebert 10.1089/ten.2006.12.2151

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Tissue engineering strategies are gathering clinical momentum in regenerative medicine and are expected to provide excellent opportunities for therapy for difficult-to-treat human pathologies. Being aware of the requirement to produce larger artificial tissue implants for clinical applications, we used microtissues, produced using gravity-enforced self-assembly of monodispersed primary cells, as minimal tissue units to generate scaffold-free vascularized artificial macrotissues in custom-shaped agarose molds. Mouse myoblast, pig and human articular-derived chondrocytes, and human myofibroblast (HMF)-composed microtissues (microm3 scale) were amalgamated to form coherent macrotissue patches (mm3 scale) of a desired shape. Macrotissues, assembled from the human umbilical vein endothelial cell (HUVEC)-coated HMF microtissues, developed a vascular system, which functionally connected to the chicken embryo's vasculature after implantation. The design of scaffold-free vascularized macrotissues is a first step toward the scale-up and production of artificial tissue implants for future tissue engineering initiatives.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
UniBE Contributor:	Djonov, Valentin Georgiev
Subjects:	600 Technology > 610 Medicine & health
ISSN:	1076-3279
ISBN:	16968156
Publisher:	Mary Ann Liebert
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:49
Last Modified:	05 Dec 2022 14:15
Publisher DOI:	10.1089/ten.2006.12.2151
PubMed ID:	16968156
Web of Science ID:	000240345800010
URI:	https://boris.unibe.ch/id/eprint/20354 (FactScience: 3642)