Design of custom-shaped vascularized tissues using microtissue spheroids as minimal building units

Kelm, Jens M; Djonov, Valentin; Ittner, Lars M; Fluri, David; Born, Walter; Hoerstrup, Simon P; Fussenegger, Martin (2006). Design of custom-shaped vascularized tissues using microtissue spheroids as minimal building units. Tissue engineering, 12(8), pp. 2151-60. Larchmont, N.Y.: Mary Ann Liebert 10.1089/ten.2006.12.2151

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Tissue engineering strategies are gathering clinical momentum in regenerative medicine and are expected to provide excellent opportunities for therapy for difficult-to-treat human pathologies. Being aware of the requirement to produce larger artificial tissue implants for clinical applications, we used microtissues, produced using gravity-enforced self-assembly of monodispersed primary cells, as minimal tissue units to generate scaffold-free vascularized artificial macrotissues in custom-shaped agarose molds. Mouse myoblast, pig and human articular-derived chondrocytes, and human myofibroblast (HMF)-composed microtissues (microm3 scale) were amalgamated to form coherent macrotissue patches (mm3 scale) of a desired shape. Macrotissues, assembled from the human umbilical vein endothelial cell (HUVEC)-coated HMF microtissues, developed a vascular system, which functionally connected to the chicken embryo's vasculature after implantation. The design of scaffold-free vascularized macrotissues is a first step toward the scale-up and production of artificial tissue implants for future tissue engineering initiatives.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy

UniBE Contributor:

Djonov, Valentin

ISSN:

1076-3279

ISBN:

16968156

Publisher:

Mary Ann Liebert

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:49

Last Modified:

17 Mar 2015 21:49

Publisher DOI:

10.1089/ten.2006.12.2151

PubMed ID:

16968156

Web of Science ID:

000240345800010

URI:

https://boris.unibe.ch/id/eprint/20354 (FactScience: 3642)

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