Cryoultramicrotomy

Vanhecke, Dimitri; Studer, Luca; Studer, Daniel (2007). Cryoultramicrotomy. In: Kuo, John (ed.) Electron Microscopy. Methods in Molecular Biology: Vol. 369 (pp. 175-197). Humana Press Inc. 10.1007/978-1-59745-294-6_9

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Cryoultramicrotomy allows the sectioning of vitrified biological samples. These biological samples are preserved at the atomic level and represent the real structure at the moment of freezing. Cryoultramicrotomy produces ultra-thin cryosections that are investigated in a cryoelectron microscope. The necessity of working during the whole preparation at temperatures less than −140°C results in some difficulties, including the cryosection transfer from the knife-edge to the electron microscropy grid; the grid handling in the cryochamber and the grid transfer into the cryoholder of the electron microscope. Furthermore, ice crystal contamination (from air humidity) can obscure the structures of interest in the sections. It is mainly know-how and experience that will prevent the contamination of ice crystals and the recrystallization of the sections during the manipulations. Here, we describe the tips, tricks, the tools, and methods that help to overcome these burdens and pave the path for successful cryoultramicrotomy.

Item Type:	Book Section (Book Chapter)
Division/Institute:	04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
UniBE Contributor:	Vanhecke, Dimitri, Studer, Daniel Franz
Subjects:	600 Technology > 610 Medicine & health
ISSN:	1064-3745
ISBN:	978-1-59745-294-6
Series:	Methods in Molecular Biology
Publisher:	Humana Press Inc.
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:49
Last Modified:	05 Dec 2022 14:15
Publisher DOI:	10.1007/978-1-59745-294-6_9
PubMed ID:	17656751
URI:	https://boris.unibe.ch/id/eprint/20373 (FactScience: 3661)