Cryoultramicrotomy

Vanhecke, Dimitri; Studer, Luca; Studer, Daniel (2007). Cryoultramicrotomy. In: Kuo, John (ed.) Electron Microscopy. Methods in Molecular Biology: Vol. 369 (pp. 175-197). Humana Press Inc. 10.1007/978-1-59745-294-6_9

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Cryoultramicrotomy allows the sectioning of vitrified biological samples. These biological samples are preserved at the atomic level and represent the real structure at the moment of freezing. Cryoultramicrotomy produces ultra-thin cryosections that are investigated in a cryoelectron microscope. The necessity of working during the whole preparation at temperatures less than −140°C results in some difficulties, including the cryosection transfer from the knife-edge to the electron microscropy grid; the grid handling in the cryochamber and the grid transfer into the cryoholder of the electron microscope. Furthermore, ice crystal contamination (from air humidity) can obscure the structures of interest in the sections. It is mainly know-how and experience that will prevent the contamination of ice crystals and the recrystallization of the sections during the manipulations. Here, we describe the tips, tricks, the tools, and methods that help to overcome these burdens and pave the path for successful cryoultramicrotomy.

Item Type:

Book Section (Book Chapter)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy

UniBE Contributor:

Vanhecke, Dimitri and Studer, Daniel Franz

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1064-3745

ISBN:

978-1-59745-294-6

Series:

Methods in Molecular Biology

Publisher:

Humana Press Inc.

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:49

Last Modified:

07 Apr 2014 00:04

Publisher DOI:

10.1007/978-1-59745-294-6_9

PubMed ID:

17656751

URI:

https://boris.unibe.ch/id/eprint/20373 (FactScience: 3661)

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