Detection of erythropoietin in exhaled breath condensate of nonhypoxic subjects using a multiplex bead array

Schumann, Christian; Triantafilou, Kathy; Krueger, Stefan; Hombach, Vinzenz; Triantafilou, Martha; Becher, Gunther; Lepper, Philipp M. (2006). Detection of erythropoietin in exhaled breath condensate of nonhypoxic subjects using a multiplex bead array. Mediators of inflammation, 2006(5), p. 18061. Sylvania, Ohio: Hindawi Publishing Corp. 10.1155/MI/2006/18061

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As a noninvasive method, exhaled breath condensate (EBC) has gained importance to improve monitoring of lung diseases and to detect biomarkers. The aim of the study was to investigate, whether erythropoietin (EPO) is detectable in EBC. EBC was collected from 22 consecutive patients as well as from healthy individuals. Using a multiplex fluorescent bead immunoassay, we detected EPO in EBC, as well as tumour necrosis factor-alpha (TNF-alpha) in 13 out of 22 patients simultaneously (EPO 0.21 +/- 0.03 in U/mL and TNF-alpha 34.6 +/- 4.2 in pg/mL, mean +/- SEM). No significant differences for EPO levels or correlation between EPO and TNF-alpha were found but TNF-alpha was significantly higher in patients with chronic obstructive pulmonary disease (COPD) than in non-COPD (obstructive sleep apnoea, OSA, and lung healthy patients). This is the first report of detection of EPO in EBC. Due to the small study size more data is needed to clarify the role of EPO in EBC.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Intensive Care, Emergency Medicine and Anaesthesiology (DINA) > Clinic of Intensive Care

UniBE Contributor:

Lepper, Philipp

ISSN:

0962-9351

ISBN:

17392570

Publisher:

Hindawi Publishing Corp.

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:49

Last Modified:

01 Jan 2015 19:55

Publisher DOI:

10.1155/MI/2006/18061

PubMed ID:

17392570

Web of Science ID:

000242587000001

BORIS DOI:

10.7892/boris.20408

URI:

https://boris.unibe.ch/id/eprint/20408 (FactScience: 3699)

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