Toxic effects of lipid-mediated gene transfer in ventral mesencephalic explant cultures

Bauer, Matthias; Kristensen, Bjarne Winther; Meyer, Morten; Gasser, Thomas; Widmer, Hans Ruedi; Zimmer, Jens; Ueffing, Marius (2006). Toxic effects of lipid-mediated gene transfer in ventral mesencephalic explant cultures. Basic & clinical pharmacology & toxicology, 98(4), pp. 395-400. Malden, Mass.: Wiley-Blackwell 10.1111/j.1742-7843.2006.pto_310.x

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Adverse effects of cDNA and oligonucleotide delivery methods have not yet been systematically analyzed. We introduce a protocol to monitor toxic effects of two non-viral lipid-based gene delivery protocols using CNS primary tissue. Cell membrane damage was monitored by quantifying cellular uptake of propidium iodide and release of cytosolic lactate dehydrogenase to the culture medium. Using a liposomal transfection reagent, cell membrane damage was already seen 24 hr after transfection. Nestin-positive target cells, which were used as morphological correlate, were severely diminished in some areas of the cultures after liposomal transfection. In contrast, the non-liposomal transfection reagent revealed no signs of toxicity. This approach provides easily accessible information of transfection-associated toxicity and appears suitable for prescreening of transfection reagents.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Neurosurgery
UniBE Contributor:	Widmer, Hans Rudolf
ISSN:	1742-7835
ISBN:	16623864
Publisher:	Wiley-Blackwell
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:50
Last Modified:	05 Dec 2022 14:15
Publisher DOI:	10.1111/j.1742-7843.2006.pto_310.x
PubMed ID:	16623864
Web of Science ID:	000236673000011
URI:	https://boris.unibe.ch/id/eprint/21059 (FactScience: 4938)