Pattern of mineralization after regenerative periodontal therapy with enamel matrix proteins

Bosshardt, Dieter; Sculean, Anton; Donos, Nikolaos; Lang, Niklaus Peter (2006). Pattern of mineralization after regenerative periodontal therapy with enamel matrix proteins. European journal of oral sciences, 114 Suppl 1, pp. 225-231. Chichester: Wiley-Blackwell 10.1111/j.1600-0722.2006.00300.x

[img] Text
j.1600-0722.2006.00300.x.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (429kB) | Request a copy

A derivative (EMD) of enamel matrix proteins (EMPs) is used for periodontal regeneration because EMPs are believed to induce the formation of acellular extrinsic fiber cementum (AEFC). Other reports, however, indicate that EMPs have osteogenic potential. The aim of this study was to characterize the nature of the tissue that forms on the root surface following application of EMD. Ten human teeth affected by periodontitis and scheduled for extraction were treated with EMD. Four to six weeks later, they were extracted and processed for analysis by light microscopy and transmission electron microscopy. Immunocytochemistry with antibodies against bone sialoprotein (BSP) and osteopontin (OPN) was performed to determine the mineralization pattern. The newly formed tissues on the root were thick and contained embedded cells. Small mineralization foci were regularly seen, and large organic matrix patches were occasionally seen, but a distinct mineralization front was lacking. While labeling for BSP was always associated with small mineralization foci and large matrix patches, OPN labeling was seen inconsistently. It is concluded that tissues resembling either cellular intrinsic fiber cementum or a type of bone were observed. The mineralization pattern mostly resembled that found in bone, except for a few areas that exhibited a hitherto undescribed mineralization pattern.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology

UniBE Contributor:

Bosshardt, Dieter; Sculean, Anton; Donos, Nikolaos and Lang, Niklaus Peter

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0909-8836

Publisher:

Wiley-Blackwell

Submitter:

Eveline Carmen Schuler

Date Deposited:

04 Oct 2013 14:51

Last Modified:

25 Jan 2017 12:17

Publisher DOI:

10.1111/j.1600-0722.2006.00300.x

PubMed ID:

16674690

Web of Science ID:

000240404800038

BORIS DOI:

10.7892/boris.21593

URI:

https://boris.unibe.ch/id/eprint/21593 (FactScience: 8085)

Actions (login required)

Edit item Edit item
Provide Feedback