A comparative analysis of phenotype expression in human osteoblasts from heterotopic ossification and normal bone

Handschin, Alexander E; Egermann, Marcus; Wedler, Volker; Trentz, Otmar; Hemmi, Sonja; Trentz, Omana A (2006). A comparative analysis of phenotype expression in human osteoblasts from heterotopic ossification and normal bone. Langenbeck's archives of surgery, 391(4), pp. 376-82. Berlin: Springer 10.1007/s00423-005-0021-5

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BACKGROUND AND AIMS: Heterotopic ossification (HO) is a pathological bone formation process in which ectopic bone is formed in soft tissue. The formation of bone depends on the expression of the osteoblast phenotype. Earlier studies have shown conflicting results on the expression of phenotype markers of cells originating from HO and normal bone. The hypothesis of the present study is that cells from HO show an altered expression of osteoblast-specific phenotype markers compared to normal osteoblasts. The aims of the study were to further characterize the expression of osteoblast phenotypemarkers and to provide a comparison with other study results. PATIENTS AND METHODS: Using an in vitro technique, reverse transcription polymerase chain reaction (RT-PCR), real-time PCR and immunohistochemistry, we compared the phenotype gene expression (type I collagen, alkaline phosphatase, Cbfa-1, osteocalcin) of osteoblasts from resected HO and normal bone (iliac crest). RESULTS: Cells from HO expressed the osteoblast phenotype (type I collagen, alkaline phosphatase) but were characterized by a depleted osteocalcin expression. The expression of Cbfa-1 (osteocalcin transcription gene) showed a large variety in our study. Preoperative radiotherapy had no effect on phenotype expression in cells from HO. CONCLUSION: Our results provide a characterization of cells originating from HO and support the thesis of an impaired osteoblast differentiation underlying the formation of HO. The transcription axis from Cbfa-1 to osteocalcin could be involved in the pathogenesis of HO.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Orthopaedic, Plastic and Hand Surgery (DOPH) > Clinic of Plastic and Hand Surgery > Plastic, Reconstructive and Aesthetic Surgery

UniBE Contributor:

Handschin, Alexander

ISSN:

1435-2443

ISBN:

16501989

Publisher:

Springer

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:51

Last Modified:

17 Mar 2015 21:55

Publisher DOI:

10.1007/s00423-005-0021-5

PubMed ID:

16501989

Web of Science ID:

000240105300012

URI:

https://boris.unibe.ch/id/eprint/21740 (FactScience: 13457)

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