A technique to detect and to quantify fasciocutaneous blood vessels in small laboratory animals ex vivo

Machens, H G; Grzybowski, S; Bucsky, B; Spanholtz, T; Niedworok, C; Maichle, A; Stöckelhuber, B; Condurache, A; Liu, F; Egana, J T; Kaun, M; Mailänder, P; Aach, T (2006). A technique to detect and to quantify fasciocutaneous blood vessels in small laboratory animals ex vivo. Journal of surgical research, 131(1), pp. 91-6. New York, N.Y.: Elsevier 10.1016/j.jss.2005.08.024

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PURPOSE: A microangiographical technique is described, which allows visualization of small and capillary blood vessels and quantification of fasciocutaneous blood vessels by means of digital computer analysis in very small laboratory animals. MATERIALS AND METHODS: The left carotid artery of 20 nu/nu mice was cannulated (26 gauge) and a mixture of gelatin, bariumsulfate, and green ink was injected according to standardized protocol. Fasciocutaneous blood vessels were visualized by digital mammography and analyzed for vessel length and vessel surface area as standardized units [SU] by computer program. RESULTS: With the described microangiography method, fasciocutaneous blood vessels down to capillary size level can be clearly visualized. Regions of interest (ROIs) can be defined and the containing vascular network quantified. Comparable results may be obtained by calculating the microvascular area index (MAI) and the microvascular length index (MLI), related to the ROIs size. Identical ROIs showed a high reproducibility for measured [SU] < 0.01 +/- 0.0012%. CONCLUSION: Combining microsurgical techniques, pharmacological knowledge, and modern digital image technology, we were able to visualize small and capillary blood vessels even in small laboratory animals. By using our own computer analytical program, quantification of vessels was reliable, highly reproducible, and fast.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Department of Orthopaedic, Plastic and Hand Surgery (DOPH) > Clinic of Plastic and Hand Surgery > Plastic, Reconstructive and Aesthetic Surgery
UniBE Contributor:	Spanholtz, Timo Alexander
ISSN:	0022-4804
ISBN:	16274694
Publisher:	Elsevier
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:51
Last Modified:	05 Dec 2022 14:16
Publisher DOI:	10.1016/j.jss.2005.08.024
PubMed ID:	16274694
Web of Science ID:	000235771200013
URI:	https://boris.unibe.ch/id/eprint/21741 (FactScience: 13480)