Kinetics of the interaction of desAABB-fibrin monomer with immobilized fibrinogen

Chtcheglova, Lilia A; Vogel, Monique; Gruber, Hermann J; Dietler, Giovanni; Haeberli, André (2006). Kinetics of the interaction of desAABB-fibrin monomer with immobilized fibrinogen. Biopolymers, 83(1), pp. 69-82. Hoboken, N.J.: John Wiley & Sons, Inc. 10.1002/bip.20529

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The soluble and stable fibrin monomer-fibrinogen complex (SF) is well known to be present in the circulating blood of healthy individuals and of patients with thrombotic diseases. However, its physiological role is not yet fully understood. To deepen our knowledge about this complex, a method for the quantitative analysis of interaction between soluble fibrin monomers and surface-immobilized fibrinogen has been established by means of resonant mirror (IAsys) and surface plasmon resonance (BIAcore) biosensors. The protocols have been optimized and validated by choosing appropriate immobilization procedures with regeneration steps and suitable fibrin concentrations. The highly specific binding of fibrin monomers to immobilized fibrin(ogen), or vice versa, was characterized by an affinity constant of approximately 10(-8)M, which accords better with the direct dissociation of fibrin triads (KD approximately 10(-8) -10(-9) M) (J. R. Shainoff and B. N. Dardik, Annals of the New York Academy of Science, 1983, Vol. 27, pp. 254-268) than with earlier estimations of the KD for the fibrin-fibrinogen complex (KD approximately 10(-6) M) (J. L. Usero, C. Izquierdo, F. J. Burguillo, M. G. Roig, A. del Arco, and M. A. Herraez, International Journal of Biochemistry, 1981, Vol. 13, pp. 1191-1196).

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Unit Childrens Hospital > Thromboselabor Kinderklinik (discontinued)

UniBE Contributor:

Haeberli, André

ISSN:

0006-3525

ISBN:

16639743

Publisher:

John Wiley & Sons, Inc.

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:52

Last Modified:

06 Dec 2013 13:45

Publisher DOI:

10.1002/bip.20529

PubMed ID:

16639743

Web of Science ID:

000239617300008

URI:

https://boris.unibe.ch/id/eprint/21942 (FactScience: 19670)

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