Endothelium-specific replacement of the connexin43 coding region by a lacZ reporter gene

Theis, M; de Wit, C; Schlaeger, TM; Eckardt, D; Krüger, O; Döring, B; Risau, W; Deutsch, U; Pohl, U; Willecke, K (2001). Endothelium-specific replacement of the connexin43 coding region by a lacZ reporter gene. Genesis, 29(1), pp. 1-13. Hoboken, N.J.: Wiley-Liss 10.1002/1526-968X(200101)29:1<1::AID-GENE1000>3.0.CO;2-0

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The murine gap junction protein connexin43 (Cx43) is expressed in blood vessels, with vastly different contribution by endothelial and smooth muscle cells. We have used the Cre recombinase under control of TIE2 transcriptional elements to inactivate a floxed Cx43 gene specifically in endothelial cells. Cre-mediated deletion led to replacement of the Cx43 coding region by a lacZ reporter gene. This allowed us to monitor the extent of deletion and to visualize the endothelial expression pattern of Cx43. We found widespread endothelial expression of the Cx43 gene during embryonic development, which became restricted largely to capillaries and small vessels in all adult organs examined. Mice lacking Cx43 in endothelium did not exhibit altered blood pressure, in contrast to mice deficient in Cx40. Our results show that lacZ activation after deletion of the target gene allows us to determine the extent of cell type-specific deletion after phenotypical investigation of the same animal.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Theodor Kocher Institute

UniBE Contributor:

Deutsch, Urban

ISSN:

1526-954X

ISBN:

11135457

Publisher:

Wiley-Liss

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:52

Last Modified:

17 Mar 2015 21:56

Publisher DOI:

10.1002/1526-968X(200101)29:1<1::AID-GENE1000>3.0.CO;2-0

PubMed ID:

11135457

Web of Science ID:

000166668100001

URI:

https://boris.unibe.ch/id/eprint/22224 (FactScience: 32302)

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