Regulation of endothelial monocyte-activating polypeptide II release by apoptosis

Knies, UE; Behrensdorf, HA; Mitchell, CA; Deutsch, U; Risau, W; Drexler, HC; Clauss, M (1998). Regulation of endothelial monocyte-activating polypeptide II release by apoptosis. Proceedings of the National Academy of Sciences of the United States of America - PNAS, 95(21), pp. 12322-7. Washington, D.C.: National Academy of Sciences NAS 10.1073/pnas.95.21.12322

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Endothelial monocyte-activating polypeptide II (EMAP II) is a proinflammatory cytokine and a chemoattractant for monocytes. We show here that, in the mouse embryo, EMAP II mRNA was most abundant at sites of tissue remodeling where many apoptotic cells could be detected by terminal deoxynucleotidyltransferase-mediated dUTP end labeling. Removal of dead cells is known to require macrophages, and these were found to colocalize with areas of EMAP II mRNA expression and programmed cell death. In cultured cells, post-translational processing of pro-EMAP II protein to the mature released EMAP II form (23 kDa) occurred coincidentally with apoptosis. Cleavage of pro-EMAP II could be abrogated in cultured cells by using a peptide-based inhibitor, which competes with the ASTD cleavage site of pro-EMAP II. Our results suggest that the coordinate program of cell death includes activation of a caspase-like activity that initiates the processing of a cytokine responsible for macrophage attraction to the sites of apoptosis.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Pre-clinic Human Medicine > Theodor Kocher Institute
UniBE Contributor:	Deutsch, Urban
ISSN:	0027-8424
ISBN:	9770485
Publisher:	National Academy of Sciences NAS
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:52
Last Modified:	05 Dec 2022 14:16
Publisher DOI:	10.1073/pnas.95.21.12322
PubMed ID:	9770485
Web of Science ID:	000076447900047
URI:	https://boris.unibe.ch/id/eprint/22228 (FactScience: 32310)