Tie2 receptor expression and phosphorylation in cultured cells and mouse tissues

Koblizek, TI; Runting, AS; Stacker, SA; Wilks, AF; Risau, W; Deutsch, U (1997). Tie2 receptor expression and phosphorylation in cultured cells and mouse tissues. European journal of biochemistry, 244(3), pp. 774-9. Cambridge: Blackwell Science 10.1111/j.1432-1033.1997.00774.x

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Accumulating experimental evidence indicates that endothelial cell growth and blood vessel morphogenesis are processes that are governed by the activity of specifically expressed receptor tyrosine kinases (RTKs). We have used two new rat monoclonal antibodies (mAbs) to study the expression and phosphorylation of one such receptor, mouse Tie2 (tyrosine kinase that contains immunoglobulin-like loops and epidermal-growth-factor-similar domains 2]), in transfected cells, endothelioma cell lines and mouse tissues. The Tie2 receptor was found to be constitutively autophosphorylated when over-expressed in COS7 cells. In contrast, the endogenous Tie2 protein was not phosphorylated in endothelioma cell lines. However, in these cell lines, Tie2 could be induced to become tyrosine phosphorylated, and this activation was found to be independent of Tie1. Studying Tie2 receptor activity during angiogenesis in mouse development, the receptor was only weakly phosphorylated in the early postnatal mouse brain whereas a stronger activation could be detected in mouse embryos at day 10.5 post coitum.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Theodor Kocher Institute

UniBE Contributor:

Deutsch, Urban

ISSN:

0014-2956

ISBN:

9108247

Publisher:

Blackwell Science

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:52

Last Modified:

04 May 2014 23:15

Publisher DOI:

10.1111/j.1432-1033.1997.00774.x

PubMed ID:

9108247

Web of Science ID:

A1997WP33100013

URI:

https://boris.unibe.ch/id/eprint/22232 (FactScience: 32318)

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