Synergistic inhibition in cell-cell fusion mediated by the matrix and nucleocapsid protein of canine distemper virus

Wiener, Dominique; Plattet, Philippe; Cherpillod, Pascal; Zipperle, Ljerka; Doherr, Marcus G; Vandevelde, Marc; Zurbriggen, Andreas (2007). Synergistic inhibition in cell-cell fusion mediated by the matrix and nucleocapsid protein of canine distemper virus. Virus research, 129(2), pp. 145-54. Amsterdam: Elsevier 10.1016/j.virusres.2007.07.004

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Canine distemper virus (CDV) causes a chronic, demyelinating, progressive or relapsing neurological disease in dogs, because CDV persists in the CNS. Persistence of virulent CDV, such as the A75/17 strain has been reproduced in cell cultures where it is associated with a non-cytolytic infection with very limited cell-cell fusion. This is in sharp contrast to attenuated CDV infection in cell cultures, such as the Onderstepoort (OP) CDV strain, which produces extensive fusion activity and cytolysis. Fusion efficiency may be determined by the structure of the viral fusion protein per se but also by its interaction with other structural proteins of CDV. This was studied by combining genes derived from persistent and non-persistent CDV strains in transient transfection experiments. It was found that fusion efficiency was markedly attenuated by the structure of the fusion protein of the neurovirulent A75/17-CDV. Moreover, we showed that the interaction of the surface glycoproteins with the M protein of the persistent strain greatly influenced fusion activity. Site directed mutagenesis showed that the c-terminus of the M protein is of particular importance in this respect. Interestingly, although the nucleocapsid protein alone did not affect F/H-induced cell-cell fusion, maximal inhibition occurred when the latter was added to combined glycoproteins with matrix protein. Thus, the present study suggests that very limited fusogenicity in virulent CDV infection, which favours persistence by limiting cell destruction involves complex interactions between all viral structural proteins.

Item Type:	Journal Article (Original Article)
Division/Institute:	05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > DVK - Clinical Research [discontinued] 05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research 05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > DKV - Clinical Neurology
UniBE Contributor:	Wiener, Dominique Judith, Plattet, Philippe, Zipperle, Ljerka, Doherr, Marcus, Vandevelde, Marc, Zurbriggen, Andreas (A)
ISSN:	0168-1702
Publisher:	Elsevier
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:52
Last Modified:	29 Mar 2023 23:32
Publisher DOI:	10.1016/j.virusres.2007.07.004
PubMed ID:	17706826
Web of Science ID:	000251111000009
URI:	https://boris.unibe.ch/id/eprint/22255 (FactScience: 33614)