Diversity in neuroanatomical distribution of abnormal prion protein in atypical scrapie

Nentwig, Alice; Oevermann, Anna; Heim, Dagmar; Botteron, Catherine; Zellweger, Karola; Drögemüller, Cord; Zurbriggen, Andreas; Seuberlich, Torsten (2007). Diversity in neuroanatomical distribution of abnormal prion protein in atypical scrapie. PLoS pathogens, 3(6), e82. San Francisco, Calif.: Public Library of Science 10.1371/journal.ppat.0030082

[img]
Preview
Text
http___www.plospathogens.org_article_fetchObject.action_uri=info_doi_10.1371_journal.ppat.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (536kB) | Preview

Scrapie is a transmissible spongiform encephalopathy (TSE) in sheep and goats. In recent years, atypical scrapie cases were identified that differed from classical scrapie in the molecular characteristics of the disease-associated pathological prion protein (PrP(sc)). In this study, we analyze the molecular and neuropathological phenotype of nine Swiss TSE cases in sheep and goats. One sheep was identified as classical scrapie, whereas six sheep, as well as two goats, were classified as atypical scrapie. The latter revealed a uniform electrophoretic mobility pattern of the proteinase K-resistant core fragment of PrP(sc) distinct from classical scrapie regardless of the genotype, the species, and the neuroanatomical structure. Remarkably different types of neuroanatomical PrP(sc) distribution were observed in atypical scrapie cases by both western immunoblotting and immunohistochemistry. Our findings indicate that the biodiversity in atypical scrapie is larger than expected and thus impacts on current sampling and testing strategies in small ruminant TSE surveillance.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > DVK - Clinical Research [discontinued]
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Institute of Genetics

UniBE Contributor:

Nentwig, Alice; Oevermann, Anna; Botteron, Catherine; Zellweger, Karola; Drögemüller, Cord; Zurbriggen, Andreas and Seuberlich, Torsten

ISSN:

1553-7366

Publisher:

Public Library of Science

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:52

Last Modified:

16 Nov 2015 15:15

Publisher DOI:

10.1371/journal.ppat.0030082

PubMed ID:

17559305

Web of Science ID:

000248511400006

BORIS DOI:

10.7892/boris.22257

URI:

https://boris.unibe.ch/id/eprint/22257 (FactScience: 33622)

Actions (login required)

Edit item Edit item
Provide Feedback