Differential expression of CD45 on canine microglial cells

Stein, V M; Baumgärtner, W; Schröder, S; Zurbriggen, Andreas; Vandevelde, Marc; Tipold, A (2007). Differential expression of CD45 on canine microglial cells. Journal of veterinary medicine. Series A - physiology, pathology, clinical medicine, 54(6), pp. 314-20. Berlin: Blackwell 10.1111/j.1439-0442.2007.00926.x

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CD45, also called leucocyte common antigen is a transmembrane protein tyrosine phosphatase on the surface of nearly all white blood cells and has a functional role in signal transduction. In the brain, the expression of CD45 can be used to distinguish microglial cells with a characteristic phenotype of CD11b/c+ and CD45(low) from other central nervous system (CNS) macrophages which show an expression of CD11b/c+ and CD45(high). In the course of pathological changes in the CNS, microglia in rodents is known to readily upregulate expression of various surface molecules, such as CD45. Understanding the mechanisms that regulate expression of surface molecules is essential to study the pathogenesis of CNS diseases. In the present study, the expression of CD45 on microglia of 42 dogs was examined ex vivo by means of flow cytometry. The dogs were classified in two groups according to the histopathological diagnosis in the CNS. All dogs without changes in the CNS (group I; n = 22) only showed low percentages of CD45+ microglial cells. In group II consisting of 20 dogs with different intracranial diseases varying results were obtained. Thirteen dogs showed a low percentage of CD45+ microglial cells whereas seven dogs exhibited high percentages of microglial cells expressing CD45. Evaluation of expression intensity in these seven dogs revealed two subpopulations of CD45+ microglial cells: a large subpopulation with CD45(low) and a small subpopulation with CD45(high). The expression intensity of CD45(high) was comparable with that of canine monocytes. It was attempted to correlate these findings to age of the animals, underlying disease, duration of clinical signs, medical treatment, occurrence of seizure activity and the expression of other surface molecules. It appeared that dogs with high percentages of CD45+ suffered from long-lasting CNS disease with seizures. In future studies, the reason and consequences for upregulated CD45 in long-lasting CNS diseases has to be further evaluated.

Item Type:	Journal Article (Original Article)
Division/Institute:	05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > DKV - Clinical Neurology 05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research
UniBE Contributor:	Zurbriggen, Andreas (A), Vandevelde, Marc
ISSN:	0931-184X
Publisher:	Blackwell
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:52
Last Modified:	29 Mar 2023 23:32
Publisher DOI:	10.1111/j.1439-0442.2007.00926.x
PubMed ID:	17650152
Web of Science ID:	000248593600007
URI:	https://boris.unibe.ch/id/eprint/22260 (FactScience: 33628)