Sendai virus RNA polymerase scanning for mRNA start sites at gene junctions

Plattet, Philippe; Strahle, Laura; le Mercier, Philippe; Hausmann, Stéphane; Garcin, Dominique; Kolakofsky, Daniel (2007). Sendai virus RNA polymerase scanning for mRNA start sites at gene junctions. Virology, 362(2), pp. 411-20. New York, N.Y.: Academic Press 10.1016/j.virol.2006.12.033

Full text not available from this repository. (Request a copy)

Mini-genomes expressing two reporter genes and a variable gene junction were used to study Sendai virus RNA polymerase (RdRp) scanning for the mRNA start signal of the downstream gene (gs2). We found that RdRp could scan the template efficiently as long as the initiating uridylate of gs2 (3' UCCCnnUUUC) was preceded by the conserved intergenic region (3' GAA) and the last 3 uridylates of the upstream gene end signal (ge1; 3' AUUCUUUUU). The end of the leader sequence (3' CUAAAA, which precedes gs1) could also be used for gene2 expression, but this sequence was considerably less efficient. Increasing the distance between ge1 and gs2 (up to 200 nt) led to the progressive loss of gene2 expression, in which half of gene2 expression was lost for each 70 nucleotides of intervening sequence. Beyond 200 nt, gene2 expression was lost more slowly. Our results suggest that there may be two populations of RdRp that scan at gene junctions, which can be distinguished by the efficiency with which they can scan the genome template for gs.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research

UniBE Contributor:

Plattet, Philippe

ISSN:

0042-6822

Publisher:

Academic Press

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:52

Last Modified:

21 Jan 2014 15:05

Publisher DOI:

10.1016/j.virol.2006.12.033

PubMed ID:

17300823

Web of Science ID:

000246791800017

URI:

https://boris.unibe.ch/id/eprint/22262 (FactScience: 33656)

Actions (login required)

Edit item Edit item
Provide Feedback