Moulin, Hervé R; Seuberlich, Torsten; Bauhofer, Oliver; Bennett, Lea C; Tratschin, Jon-Duri; Hofmann, Martin A; Ruggli, Nicolas (2007). Nonstructural proteins NS2-3 and NS4A of classical swine fever virus: essential features for infectious particle formation. Virology, 365(2), pp. 376-89. New York, N.Y.: Academic Press 10.1016/j.virol.2007.03.056
Full text not available from this repository.The nonstructural protein NS2-3 of pestiviruses undergoes tightly regulated processing. For bovine viral diarrhea virus it was shown that uncleaved NS2-3 is required for infectious particle formation while cleaved NS3 is essential for genome replication. To further investigate the functions of NS2-3 and NS4A in the pestivirus life cycle, we established T7 RNA polymerase-dependent trans-complementation for p7-NS2-3-4A of classical swine fever virus (CSFV). Expression of NS2-3 and NS4A in trans restored the production of infectious particles from genomes lacking NS2-3 expression. Co-expression of cleaved NS4A was essential. None of the enzymatic activities harbored by NS2-3 were required for infectious particle formation. Importantly, expression of uncleavable NS2-3 together with NS4A rescued infectious particles from a genome lacking NS2, demonstrating that cleaved NS2 per se has no additional essential function. These data indicate that NS2-3 and NS3, each in association with NS4A, have independent functions in the CSFV life cycle.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research |
UniBE Contributor: |
Seuberlich, Torsten |
ISSN: |
0042-6822 |
Publisher: |
Academic Press |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 14:52 |
Last Modified: |
05 Dec 2022 14:16 |
Publisher DOI: |
10.1016/j.virol.2007.03.056 |
PubMed ID: |
17482232 |
Web of Science ID: |
000248985200014 |
URI: |
https://boris.unibe.ch/id/eprint/22268 (FactScience: 33745) |
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