Validation and diagnostic efficacy of a TaqMan real-time PCR for the detection of Mycoplasma conjunctivae in the eyes of infected Caprinae

Vilei, Edy M; Bonvin-Klotz, Laetitia; Zimmermann, Liza; Ryser-Degiorgis, Marie-Pierre; Giacometti, Marco; Frey, Joachim (2007). Validation and diagnostic efficacy of a TaqMan real-time PCR for the detection of Mycoplasma conjunctivae in the eyes of infected Caprinae. Journal of microbiological methods, 70(2), pp. 384-6. Amsterdam: Elsevier 10.1016/j.mimet.2007.05.007

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Infectious keratoconjunctivitis (IKC), caused by Mycoplasma conjunctivae, is a highly contagious ocular disease in Caprinae. To detect rapidly and sensitively M. conjunctivae from individual conjunctival swabs of infected domestic and wild animals, a specific real-time PCR was developed using an lppS-directed hydrolysis probe in a TaqMan platform.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Center for Fish and Wildlife Health (FIWI)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Anatomy

UniBE Contributor:

Vilei, Edi; Bonvin, Laetitia Anne; Zimmermann, Liza; Ryser, Marie Pierre and Frey, Joachim

Subjects:

600 Technology > 630 Agriculture

ISSN:

0167-7012

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:53

Last Modified:

22 Mar 2016 15:49

Publisher DOI:

10.1016/j.mimet.2007.05.007

PubMed ID:

17570547

Web of Science ID:

000248523900021

URI:

https://boris.unibe.ch/id/eprint/22355 (FactScience: 34292)

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