Turning placenta into brain: placental mesenchymal stem cells differentiate into neurons and oligodendrocytes

Portmann-Lanz, C Bettina; Schoeberlein, Andreina; Portmann, Reto; Mohr, Stefan; Rollini, Pierre; Sager, Ruth; Surbek, Daniel V (2010). Turning placenta into brain: placental mesenchymal stem cells differentiate into neurons and oligodendrocytes. American journal of obstetrics and gynecology, 202(3), 294.e1-294.e11. Orlando, Fla.: Elsevier 10.1016/j.ajog.2009.10.893

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Objective We aimed to induce neural stem (NSC) and progenitor cells (NPC) from human placental tissues. Study Design Placental stem cells from first-trimester placental chorionic villi and term chorion were isolated. Neural differentiation was initiated with plating on collagen, retinoic acid, and/or human brain-derived neurotrophic factor and epidermal and fibroblast growth factor. Differentiation into neurons, oligodendrocytes, and astrocytes was monitored by immunohistochemistry. Two-dimensional polyacrylamide gel electrophoresis, high-performance liquid chromatography, and tandem mass spectrometry were used to identify proteins involved in the differentiation. Results Differentiated cells were mostly immediately postmitotic with some more but not fully mature postmitotic neurons. Neurons had dopaminergic or serotonergic character. Some cells differentiated into predominantly immature oligodendrocytes. Upon differentiation, neuron-specific proteins were up-regulated, whereas placental proteins were reduced. Conclusion Stem cells derived from human placenta can be differentiated into neural progenitors.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Gynaecology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Unit Childrens Hospital > Forschungsgruppe Pränatale Medizin

UniBE Contributor:

Schoeberlein, Andreina; Mohr, Stefan and Surbek, Daniel

Subjects:

500 Science
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0002-9378

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:07

Last Modified:

26 Oct 2019 07:12

Publisher DOI:

10.1016/j.ajog.2009.10.893

PubMed ID:

20060088

Web of Science ID:

000275516900031

BORIS DOI:

10.7892/boris.225

URI:

https://boris.unibe.ch/id/eprint/225 (FactScience: 197000)

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