Intra-arterial injection of neural stem cells using a microneedle technique does not cause microembolic strokes

Chua, Joshua Y; Pendharkar, Arjun V; Wang, Nancy; Choi, Raymond; Andres, Robert H; Gaeta, Xavier; Zhang, Jian; Moseley, Mike E; Guzman, Raphael (2011). Intra-arterial injection of neural stem cells using a microneedle technique does not cause microembolic strokes. Journal of cerebral blood flow and metabolism, 31(5), pp. 1263-71. New York, N.Y.: Nature Publishing Group 10.1038/jcbfm.2010.213

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Intra-arterial (IA) injection represents an experimental avenue for minimally invasive delivery of stem cells to the injured brain. It has however been reported that IA injection of stem cells carries the risk of reduction in cerebral blood flow (CBF) and microstrokes. Here we evaluate the safety of IA neural progenitor cell (NPC) delivery to the brain. Cerebral blood flow of rats was monitored during IA injection of single cell suspensions of NPCs after stroke. Animals received 1 × 10(6) NPCs either injected via a microneedle (microneedle group) into the patent common carotid artery (CCA) or via a catheter into the proximally ligated CCA (catheter group). Controls included saline-only injections and cell injections into non-stroked sham animals. Cerebral blood flow in the microneedle group remained at baseline, whereas in the catheter group a persistent (15 minutes) decrease to 78% of baseline occurred (P<0.001). In non-stroked controls, NPCs injected via the catheter method resulted in higher levels of Iba-1-positive inflammatory cells (P=0.003), higher numbers of degenerating neurons as seen in Fluoro-Jade C staining (P<0.0001) and ischemic changes on diffusion weighted imaging. With an appropriate technique, reduction in CBF and microstrokes do not occur with IA transplantation of NPCs.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Neurosurgery

UniBE Contributor:

Andres, Robert

ISSN:

0271-678X

Publisher:

Nature Publishing Group

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:12

Last Modified:

17 Mar 2015 19:13

Publisher DOI:

10.1038/jcbfm.2010.213

PubMed ID:

21157474

Web of Science ID:

000290090700010

URI:

https://boris.unibe.ch/id/eprint/2276 (FactScience: 204671)

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