Development of a highly sensitive and specific assay to detect Staphylococcus aureus in bovine mastitic milk

Graber, H U; Casey, M G; Naskova, J; Steiner, A; Schaeren, W (2007). Development of a highly sensitive and specific assay to detect Staphylococcus aureus in bovine mastitic milk. Journal of dairy science, 90(10), pp. 4661-9. Savoy, Ill.: American Dairy Science Association 10.3168/jds.2006-902

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Diagnosis of udder infections with Staphylococcus aureus by bacteriological milk testing of quarter milk samples is often not satisfactory. To get reliable results, repeated sampling is necessary, which is normally too expensive. Therefore, we developed a test that allows the highly specific detection of Staph. aureus in bovine milk samples at very low concentrations. It is based on a fast procedure to prepare bacteria from milk, followed by DNA extraction and quantitative PCR. The whole analysis is done within 5 h. For clinical milk samples, the analytical sensitivity of the assay was 50.7 times and 507 times higher than conventional bacteriology with 100 and 10 microL, respectively. The diagnostic specificity was 100%. The test is further characterized by a low intra- and interassay variability as well as by a good recovery of Staph. aureus from raw milk. Furthermore, a high correlation (R = 0.925) between the agar plate counts and the quantitative PCR methodology over the whole range of measurement was found. In addition, our test revealed considerably more positive results than bacteriology. Due to its favorable properties, the assay might become an important diagnostic tool in the context of bovine mastitis caused by Staph. aureus.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Clinic for Ruminants
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)

UniBE Contributor:

Graber, Hans Ulrich; Naskova, Javorka and Steiner, Adrian

ISSN:

0022-0302

Publisher:

American Dairy Science Association

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:54

Last Modified:

21 Jan 2014 15:05

Publisher DOI:

10.3168/jds.2006-902

PubMed ID:

17881687

Web of Science ID:

000249583700017

URI:

https://boris.unibe.ch/id/eprint/22809 (FactScience: 37074)

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