Comparison of five diagnostic methods for detecting bovine viral diarrhea virus infection in calves

Hilbe, Monika; Stalder, Hanspeter; Peterhans, Ernst; Haessig, Michael; Nussbaumer, Marlies; Egli, Christoph; Schelp, Christian; Zlinszky, Kati; Ehrensperger, Felix (2007). Comparison of five diagnostic methods for detecting bovine viral diarrhea virus infection in calves. Journal of veterinary diagnostic investigation, 19(1), pp. 28-34. Columbia, Mo.: American Association of Veterinary Laboratory Diagnosticians AAVLD 10.1177/104063870701900105

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Five diagnostic techniques performed on skin biopsies (shoulder region) and/or serum were compared for detection of bovine viral diarrhea virus infection in 224 calves 0-3 months of age, 23 calves older than 3 months but younger than 7 months, and 11 cattle older than 7 months. The diagnostic methods used were immunohistochemistry (IHC), 2 commercial antigen ELISAs, 1 commercial antibody ELISA, and real-time RT-PCR. Results of 249 out of 258 skin and serum samples were identical and correlated within the 3 antigen detection methods and the real-time RT-PCR used. Twenty-six of these 249 samples were BVDV-positive with all antigen detection methods and the real-time RT-PCR. Nine out of 258 samples yielding discordant results were additionally examined by RT-PCR, RT-PCR Reamplification (ReA), and antigen ELISA I on serum and by immunohistochemistry on formalin fixed and paraffin-embedded skin biopsies. Virus isolation and genotyping was performed as well on these discordant samples. In 3 cases, transiently infected animals were identified. Two samples positive by real-time RT-PCR were interpreted as false positive and were ascribed to cross-contamination. The antigen ELISA II failed to detect 2 BVDV-positive calves due to the presence of maternal antibodies; the cause of 2 false-positive cases in this ELISA remained undetermined. Only persistently infected animals were identified in skin samples by IHC or antigen ELISA I. The 3 antigen detection methods and the real-time RT-PCR used in parallel had a high correlation rate (96.5%) and similar sensitivity and specificity values.

Item Type:	Journal Article (Original Article)
Division/Institute:	05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
UniBE Contributor:	Stalder, Hanspeter, Peterhans, Ernst
ISSN:	1040-6387
Publisher:	American Association of Veterinary Laboratory Diagnosticians AAVLD
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:54
Last Modified:	05 Dec 2022 14:16
Publisher DOI:	10.1177/104063870701900105
PubMed ID:	17459829
Web of Science ID:	000245657200005
URI:	https://boris.unibe.ch/id/eprint/22887 (FactScience: 37589)