Telomere length in paroxysmal nocturnal hemoglobinuria correlates with clone size

Baerlocher, Gabriela M; Sloand, Elaine M; Young, Neal S; Lansdorp, Peter M (2007). Telomere length in paroxysmal nocturnal hemoglobinuria correlates with clone size. Experimental hematology, 35(12), pp. 1777-81. New York, N.Y.: Elsevier 10.1016/j.exphem.2007.06.010

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OBJECTIVE: To study if telomere length can be used as a surrogate marker for the mitotic history in normal and affected hematopoietic cells from patients with paroxysmal nocturnal hemoglobinuria (PNH). METHODS: The telomere length was measured by automated multicolor flow fluorescence in situ hybridization in glycosyl-phosphatidyl-inositol anchored protein (GPI)-negative and GPI-positive peripheral blood leukocytes. Eleven patients were studied, two with predominantly hemolytic PNH and nine with PNH associated with marrow failure. RESULTS: Telomere length in GPI-negative cells was significantly shorter than in GPI-positive cells of the same patient (p < 0.01, n = 11). The difference in telomere length (telomere length in GPI-positive minus telomere length in GPI-negative cells) correlated with the percentage of GPI-negative white blood cells. CONCLUSION: Our results support the hypothesis that telomere length is correlated to the replicative history of GPI-positive and GPI-negative cells and warrant further studies of telomere length in relation to disease progression in PNH.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Haematology and Central Haematological Laboratory

UniBE Contributor:

Baerlocher, Gabriela M.

ISSN:

0301-472X

ISBN:

17697745

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:54

Last Modified:

04 May 2014 23:15

Publisher DOI:

10.1016/j.exphem.2007.06.010

PubMed ID:

17697745

Web of Science ID:

000251557600003

URI:

https://boris.unibe.ch/id/eprint/22891 (FactScience: 37601)

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