Efficient transformation of Lactococcus lactis IL1403 and generation of knock-out mutants by homologous recombination

Gerber, Simon D; Solioz, Marc (2007). Efficient transformation of Lactococcus lactis IL1403 and generation of knock-out mutants by homologous recombination. Journal of basic microbiology, 47(3), pp. 281-6. Weinheim: Wiley-VCH 10.1002/jobm.200610297

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Lactococcus lactis IL1403 is a Gram-positive bacterium of great biotechnological interest for food grade applications. Its use is however hampered by the difficulty to efficiently transform this strain. We here describe a detailed, optimized electrotransformation protocol which yields a transformation efficiency of 10(6) cfu/microg of DNA with the two E. coli Gram-positive shuttle vectors pC3 and pVA838. The utility of the protocol was demonstrated by the generation of single- and double-knock-out mutants by homologous recombination.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Center of Regenerative Medicine for Skeletal Tissues (discontinued)
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Center of Regenerative Medicine for Skeletal Tissues (discontinued)

04 Faculty of Medicine > Service Sector > Institute of Clinical Pharmacology and Visceral Research (discontinued)

UniBE Contributor:

Gerber, Simon and Solioz, Marc

ISSN:

0233-111X

ISBN:

17518422

Publisher:

Wiley-VCH

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:54

Last Modified:

04 May 2014 23:15

Publisher DOI:

10.1002/jobm.200610297

PubMed ID:

17518422

Web of Science ID:

000247297200010

URI:

https://boris.unibe.ch/id/eprint/22929 (FactScience: 37757)

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