Efficient transformation of Lactococcus lactis IL1403 and generation of knock-out mutants by homologous recombination

Gerber, Simon D; Solioz, Marc (2007). Efficient transformation of Lactococcus lactis IL1403 and generation of knock-out mutants by homologous recombination. Journal of basic microbiology, 47(3), pp. 281-6. Weinheim: Wiley-VCH 10.1002/jobm.200610297

Full text not available from this repository.

Lactococcus lactis IL1403 is a Gram-positive bacterium of great biotechnological interest for food grade applications. Its use is however hampered by the difficulty to efficiently transform this strain. We here describe a detailed, optimized electrotransformation protocol which yields a transformation efficiency of 10(6) cfu/microg of DNA with the two E. coli Gram-positive shuttle vectors pC3 and pVA838. The utility of the protocol was demonstrated by the generation of single- and double-knock-out mutants by homologous recombination.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Center of Regenerative Medicine for Skeletal Tissues [discontinued] 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Center of Regenerative Medicine for Skeletal Tissues [discontinued] 04 Faculty of Medicine > Service Sector > Institute of Clinical Pharmacology and Visceral Research [discontinued]
UniBE Contributor:	Gerber, Simon (B), Solioz, Marc
ISSN:	0233-111X
ISBN:	17518422
Publisher:	Wiley-VCH
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:54
Last Modified:	29 Mar 2023 23:32
Publisher DOI:	10.1002/jobm.200610297
PubMed ID:	17518422
Web of Science ID:	000247297200010
URI:	https://boris.unibe.ch/id/eprint/22929 (FactScience: 37757)