Stimulation-specific contribution of p38 and JNK to IFN-beta gene expression in human macrophages

Reimer, Thornik; Schweizer, Matthias; Jungi, Thomas W (2007). Stimulation-specific contribution of p38 and JNK to IFN-beta gene expression in human macrophages. Journal of interferon & cytokine research, 27(9), pp. 751-5. New York, N.Y.: Mary Ann Liebert 10.1089/jir.2007.0024

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Induction of interferon-beta (IFN-beta) gene expression is a tightly regulated process, and a plethora of studies identified the signal transduction pathway TANK-binding kinase-1 (TBK-1)/IFN regulatory factor-3 (IRF-3) as essential to the induction of IFN-beta gene expression. Data regarding the role of p38 and JNK are rare, however. We investigated the contribution of these kinases to IFN-beta expression in human macrophages treated with poly(I:C), lipopolysaccharide (LPS), Sendai virus, or vesicular stomatitis virus (VSV). We found that all the stimuli induced IFN-beta mRNA, albeit to a different extent. Whereas LPS and VSV induced the phosphorylation of p38 and JNK, neither poly(I:C) nor Sendai virus led to the detection of phosphospecific signals. When inhibiting p38, a VSV-triggered IFN-beta mRNA response was inhibited, whereas inhibiting JNK suppressed an LPS-triggered response, but only when macrophages were primed with IFN-gamma. Neither poly(I:C)-induced nor Sendai virus-induced IFN-beta mRNA expression was affected when p38 and JNK were inhibited. Collectively, the data show that the contribution of p38 and JNK to the expression of IFN-beta occurs in a stimulation-specific manner in human macrophages.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology

UniBE Contributor:

Reimer, Thornik; Schweizer, Matthias and Jungi, Thomas

ISSN:

1079-9907

Publisher:

Mary Ann Liebert

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:54

Last Modified:

21 Jan 2014 15:05

Publisher DOI:

10.1089/jir.2007.0024

PubMed ID:

17892396

Web of Science ID:

000249695400002

URI:

https://boris.unibe.ch/id/eprint/22936 (FactScience: 37859)

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