A reservoir of Moraxella catarrhalis in human pharyngeal lymphoid tissue

Heiniger, Nadja; Spaniol, Violeta; Troller, Rolf; Vischer, Mattheus; Aebi, Christoph (2007). A reservoir of Moraxella catarrhalis in human pharyngeal lymphoid tissue. Journal of infectious diseases, 196(7), pp. 1080-7. Cary, N.C.: The University of Chicago Press 10.1086/521194

[img]
Preview
Text
196-7-1080.pdf - Published Version
Available under License Publisher holds Copyright.

Download (650kB) | Preview

BACKGROUND: Early exposure of infants and long-term immunity suggest that colonization with Moraxella catarrhalis is more frequent than is determined by routine culture. We characterized a reservoir of M. catarrhalis in pharyngeal lymphoid tissue. METHODS: Tissue from 40 patients (median age, 7.1 years) undergoing elective tonsillectomy and/or adenoidectomy was analyzed for the presence of M. catarrhalis by culture, real-time DNA and RNA polymerase chain reaction (PCR), immunohistochemical analysis (IHC), and fluorescent in situ hybridization (FISH). Histologic sections were double stained for M. catarrhalis and immune cell markers, to characterize the tissue distribution of the organism. Intracellular bacteria were identified using confocal laser scanning microscopy (CLSM). RESULTS: Twenty-nine (91%) of 32 adenoids and 17 (85%) of 20 tonsils were colonized with M. catarrhalis. Detection rates for culture, DNA PCR, RNA PCR, IHC, and FISH were 7 (13%) of 52, 10 (19%) of 52, 21 (41%) of 51, 30 (61%) of 49, and 42 (88%) of 48, respectively (P<.001). Histologic analysis identified M. catarrhalis in crypts, intraepithelially, subepithelially, and (using CLSM) intracellularly. M. catarrhalis colocalized with macrophages and B cells in lymphoid follicles. CONCLUSIONS: Colonization by M. catarrhalis is more frequent than is determined by surface culture, because the organism resides both within and beneath the epithelium and invades host cells.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Ear, Nose and Throat Disorders (ENT)
04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Paediatric Medicine

UniBE Contributor:

Vischer, Mattheus and Aebi, Christoph

ISSN:

0022-1899

ISBN:

17763332

Publisher:

The University of Chicago Press

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:55

Last Modified:

08 Nov 2019 12:47

Publisher DOI:

10.1086/521194

PubMed ID:

17763332

Web of Science ID:

000249572500021

BORIS DOI:

10.7892/boris.23376

URI:

https://boris.unibe.ch/id/eprint/23376 (FactScience: 41652)

Actions (login required)

Edit item Edit item
Provide Feedback