Real-time broad-range PCR versus blood culture. A prospective pilot study in pediatric cancer patients with fever and neutropenia

Ammann, Roland A; Zucol, Franziska; Aebi, Christoph; Niggli, Felix K; Kühne, Thomas; Nadal, David (2007). Real-time broad-range PCR versus blood culture. A prospective pilot study in pediatric cancer patients with fever and neutropenia. Supportive care in cancer, 15(6), pp. 637-41. Berlin: Springer-Verlag 10.1007/s00520-007-0235-x

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MATERIALS AND METHODS: In a pilot study, results of real-time broad-range (16S rRNA) polymerase chain reaction (PCR) performed on 45 blood samples of pediatric cancer patients with fever and neutropenia were compared with blood culture results. RESULTS: The PCR assay used, having proven a high sensitivity in artificially spiked blood samples, was positive in only three of ten blood culture-positive samples, and it was positive in 10 of 35 (29%) culture-negative samples. CONCLUSION: This broad-range PCR assay, which may identify not-grown bacteria potentially contributing to fever, needs improvement in sensitivity, and different reasons for positive PCR in negative blood culture samples need to be assessed before clinical application.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Paediatric Medicine

UniBE Contributor:

Ammann, Roland and Aebi, Christoph

ISSN:

0941-4355

ISBN:

17333292

Publisher:

Springer-Verlag

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:55

Last Modified:

26 Jun 2018 15:34

Publisher DOI:

10.1007/s00520-007-0235-x

PubMed ID:

17333292

Web of Science ID:

000247531600008

BORIS DOI:

10.7892/boris.23380

URI:

https://boris.unibe.ch/id/eprint/23380 (FactScience: 41660)

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