Mani, Bernhard; Gerber, Marco; Lieby, Patricia; Boschetti, Nicola; Kempf, Christoph; Ros, Carlos (2007). Molecular mechanism underlying B19 virus inactivation and comparison to other parvoviruses. Transfusion, 47(10), pp. 1765-1774. Malden, Mass.: Wiley-Blackwell 10.1111/j.1537-2995.2007.01393.x
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BACKGROUND: B19 virus (B19V) is a human patho-gen frequently present in blood specimens. Transmis-sion of the virus occurs mainly via the respiratory route,but it has also been shown to occur through the admin-istration of contaminated plasma-derived products.Parvoviridaeare highly resistant to physicochemicaltreatments; however, B19V is more vulnerable than therest of parvoviruses. The molecular mechanism govern-ing the inactivation of B19V and the reason for itshigher vulnerability remain unknown.
STUDY DESIGN AND METHODS: After inactivation ofB19V by wet heat and low pH, the integrity of the viralcapsid was examined by immunoprecipitation with twomonoclonal antibodies directed to the N-terminal of VP1and to a conformational epitope in VP2. The accessibil-ity of the viral DNA was quantitatively analyzed bya hybridization-extension assay and by nucleasetreatment.
RESULTS: The integrity of the viral particles was main-tained during the inactivation procedure; however, thecapsids became totally depleted of viral DNA. TheDNA-depleted capsids, although not infectious, wereable to attach to target cells. Comparison studies withother members of theParvoviridaefamily revealed aremarkable instability of B19V DNA in its encapsidatedstate.
CONCLUSION: Inactivation of B19V by heat or low pHis not mediated by capsid disintegration but by the con-version of the infectious virions into DNA-depletedcapsids. The high instability of the viral DNA in itsencapsidated state is an exclusive feature of B19V,which explains its lower resistance to inactivationtreatments.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP) |
UniBE Contributor: |
Mani, Bernhard, Kempf, Christoph (B), Ros Bascunana, Carlos |
Subjects: |
500 Science > 570 Life sciences; biology 500 Science > 540 Chemistry |
ISSN: |
0041-1132 |
Publisher: |
Wiley-Blackwell |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 14:55 |
Last Modified: |
29 Mar 2023 23:33 |
Publisher DOI: |
10.1111/j.1537-2995.2007.01393.x |
PubMed ID: |
17880601 |
Web of Science ID: |
000249665300007 |
BORIS DOI: |
10.48350/23499 |
URI: |
https://boris.unibe.ch/id/eprint/23499 (FactScience: 42035) |
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