Distribution of lactate dehydrogenase in healthy and degenerative canine stifle joint cartilage

Walter, E; Spreng, D; Schmöckel, S; Schawalder, P; Tschudi, P; Friess, AE; Stoffel, Michael Hubert (2007). Distribution of lactate dehydrogenase in healthy and degenerative canine stifle joint cartilage. Histochemistry and cell biology, 128(1), pp. 7-18. Berlin: Springer-Verlag 10.1007/s00418-007-0287-5

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In dogs, degenerative joint diseases (DJD) have been shown to be associated with increased lactate dehydrogenase (LDH) activity in the synovial fluid. The goal of this study was to examine healthy and degenerative stifle joints in order to clarify the origin of LDH in synovial fluid. In order to assess the distribution of LDH, cartilage samples from healthy and degenerative knee joints were investigated by means of light and transmission electron microscopy in conjunction with immunolabeling and enzyme cytochemistry. Morphological analysis confirmed DJD. All techniques used corroborated the presence of LDH in chondrocytes and in the interterritorial matrix of healthy and degenerative stifle joints. Although enzymatic activity of LDH was clearly demonstrated in the territorial matrix by means of the tetrazolium-formazan reaction, immunolabeling for LDH was missing in this region. With respect to the distribution of LDH in the interterritorial matrix, a striking decrease from superficial to deeper layers was present in healthy dogs but was missing in affected joints. These results support the contention that LDH in synovial fluid of degenerative joints originates from cartilage. Therefore, we suggest that (1) LDH is transferred from chondrocytes to ECM in both healthy dogs and dogs with degenerative joint disease and that (2) in degenerative joints, LDH is released from chondrocytes and the ECM into synovial fluid through abrasion of cartilage as well as through enhanced diffusion as a result of increased water content and degradation of collagen.

Item Type:

Journal Article (Original Article)


05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Anatomy
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > DKV - Central Clinical Laboratory

UniBE Contributor:

Walter, Eveline; Spreng, David; Schawalder, Peter; Tschudi, Peter Rudolf and Stoffel, Michael Hubert


500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture








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Date Deposited:

04 Oct 2013 14:55

Last Modified:

25 Oct 2019 08:40

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https://boris.unibe.ch/id/eprint/23528 (FactScience: 42211)

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