Muscle transcriptome adaptations with mild eccentric ergometer exercise

Klossner, Stephan; Däpp, Christoph; Schmutz, Silvia; Vogt, Michael; Hoppeler, Hans; Flück, Martin (2007). Muscle transcriptome adaptations with mild eccentric ergometer exercise. Pflügers Archiv : European journal of physiology, 455(3), pp. 555-62. Berlin: Springer-Verlag 10.1007/s00424-007-0303-6

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The muscle has a wide range of possibilities to adapt its phenotype. Repetitive submaximal concentric exercise (i.e., shortening contractions) mainly leads to adaptations of muscle oxidative metabolism and endurance while eccentric exercise (i.e., lengthening contractions) results in muscle growth and gain of muscle strength. Modified gene expression is believed to mediate these exercise-specific muscle adjustments. In the present study, early alterations of the gene expression signature were monitored by a muscle-specific microarray. Transcript profiling was performed on muscle biopsies of vastus lateralis obtained from six male subjects before and in a 24-h time course after a single bout of mild eccentric ergometer exercise. The eccentric exercise consisted of 15 min of eccentric cycling at 50% of the individual maximal concentric power output leading to muscle soreness (5.9 on a 0-10 visual analogue scale) and limited muscle damage (1.7-fold elevated creatine kinase activity). Muscle impairment was highlighted by a transient reduction in jumping height after the eccentric exercise. On the gene expression level, we observed a general early downregulation of detected transcripts, followed by a slow recovery close to the control values within the first 24 h post exercise. Only very few regulatory factors were increased. This expression signature is different from the signature of a previously published metabolic response after an intensive endurance-type concentric exercise as well as after maximal eccentric exercise. This is the first description of the time course of changes in gene expression as a consequence of a mild eccentric stimulus.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Functional Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy

UniBE Contributor:

Klossner, Stephan; Däpp, Christoph; Vogt, Michael; Hoppeler, Hans-Heinrich and Flück, Martin

ISSN:

0031-6768

ISBN:

17701424

Publisher:

Springer-Verlag

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:55

Last Modified:

26 Jun 2018 15:05

Publisher DOI:

10.1007/s00424-007-0303-6

PubMed ID:

17701424

Web of Science ID:

000250380800013

BORIS DOI:

10.7892/boris.23530

URI:

https://boris.unibe.ch/id/eprint/23530 (FactScience: 42217)

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