Impact of preservation solution on the extent of blood-air barrier damage and edema formation in experimental lung transplantation

Mühlfeld, Christian; Müller, Katharina; Pallesen, Lars-Peder; Sandhaus, Tim; Madershahian, Navis; Richter, Joachim; Wahlers, Thorsten; Wittwer, Thorsten; Ochs, Matthias (2007). Impact of preservation solution on the extent of blood-air barrier damage and edema formation in experimental lung transplantation. Anatomical record: advances in integrative anatomy and evolutionary biology, 290(5), pp. 491-500. Hoboken, N.J.: John Wiley & Sons, Inc. 10.1002/ar.20518

Full text not available from this repository. (Request a copy)

A major aim in lung transplantation is to prevent the loss of structural integrity due to ischemia and reperfusion (I/R) injury. Preservation solutions protect the lung against I/R injury to a variable extent. We compared the influence of two extracellular-type preservation solutions (Perfadex, or PX, and Celsior, or CE) on the morphological alterations induced by I/R. Pigs were randomly assigned to sham (n = 4), PX (n = 5), or CE (n = 2) group. After flush perfusion with PX or CE, donor lungs were excised and stored for 27 hr at 4 degrees C. The left donor lung was implanted into the recipient, reperfused for 6 hr, and, afterward, prepared for light and electron microscopy. Intra-alveolar, septal, and peribronchovascular edema as well as the integrity of the blood-air barrier were determined stereologically. Intra-alveolar edema was more pronounced in CE (219.80 +/- 207.55 ml) than in PX (31.46 +/- 15.75 ml). Peribronchovascular (sham: 13.20 +/- 4.99 ml; PX: 15.57 +/- 5.53 ml; CE: 31.56 +/- 5.78 ml) and septal edema (thickness of alveolar septal interstitium, sham: 98 +/- 33 nm; PX: 84 +/- 8 nm; CE: 249 +/- 85 nm) were only found in CE. The blood-air barrier was similarly well preserved in sham and PX but showed larger areas of swollen and fragmented epithelium or endothelium in CE. The present study shows that Perfadex effectively prevents intra-alveolar, septal, and peribronchovascular edema formation as well as injury of the blood-air barrier during I/R. Celsior was not effective in preserving the lung from morphological I/R injury.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Topographical and Clinical Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy

UniBE Contributor:

Mühlfeld, Christian and Ochs, Matthias

ISSN:

1932-8486

ISBN:

17377949

Publisher:

John Wiley & Sons, Inc.

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:55

Last Modified:

06 Dec 2013 13:47

Publisher DOI:

10.1002/ar.20518

PubMed ID:

17377949

Web of Science ID:

000246275700008

URI:

https://boris.unibe.ch/id/eprint/23542 (FactScience: 42258)

Actions (login required)

Edit item Edit item
Provide Feedback