Construction of skeletal myoblast-based polyurethane scaffolds for myocardial repair

Siepe, Matthias; Giraud, Marie-Noelle; Liljensten, Elisabeth; Nydegger, Urs; Menasche, Philippe; Carrel, Thierry; Tevaearai, Hendrik T (2007). Construction of skeletal myoblast-based polyurethane scaffolds for myocardial repair. Artificial organs, 31(6), pp. 425-33. Malden, Mass.: Wiley-Blackwell 10.1111/j.1525-1594.2007.00385.x

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Intramyocardial transplantation of skeletal myoblasts augments postinfarction cardiac function. However, poor survival of injected cells limits this therapy. It is hypothesized that implantation of myoblast-based scaffolds would result in greater cell survival. Rat skeletal myoblasts were seeded on highly porous polyurethane (PU) scaffolds (7.5 x 7.5 x 2.0 mm). The effect of several scaffold pretreatments, initial cell densities, and culture periods was tested by DNA-based cell count and viability assessment. Seeded PU scaffolds were implanted on infarcted hearts and immunohistology was performed 4 weeks later. Precoating with laminin allowed the most favorable cell attachment. An initial inoculation with 5 x 10(6) cells followed by a 15-day culture period resulted in optimal myoblast proliferation. Four weeks after their implantation in rats, numerous myoblasts were found throughout the seeded patches although no sign of differentiation could be observed. This myoblast seeding technique on PU allows transfer of a large number of living myoblasts to a damaged myocardium.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Heart Surgery
UniBE Contributor:	Giraud, Marie-Noelle, Nydegger, Urs, Carrel, Thierry, Tevaearai, Hendrik
ISSN:	0160-564X
ISBN:	17537054
Publisher:	Wiley-Blackwell
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:56
Last Modified:	27 Feb 2024 14:29
Publisher DOI:	10.1111/j.1525-1594.2007.00385.x
PubMed ID:	17537054
Web of Science ID:	000246788500002
URI:	https://boris.unibe.ch/id/eprint/23955 (FactScience: 45416)