Observation and identification of metabolites emerging during postmortem decomposition of brain tissue by means of in situ 1H-magnetic resonance spectroscopy

Ith, Michael; Bigler, Peter; Scheurer, Eva; Kreis, Roland; Hofmann, Lucie; Dirnhofer, Richard; Bösch, Christoph Hans (2002). Observation and identification of metabolites emerging during postmortem decomposition of brain tissue by means of in situ 1H-magnetic resonance spectroscopy. Magnetic resonance in medicine, 48(5), pp. 915-20. New York, N.Y.: Wiley-Liss 10.1002/mrm.10294

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Postmortem decomposition of brain tissue was investigated by (1)H-magnetic resonance spectroscopy (MRS) in a sheep head model and selected human cases. Aiming at the eventual estimation of postmortem intervals in forensic medicine, this study focuses on the characterization and identification of newly observed metabolites. In situ single-voxel (1)H-MRS at 1.5 T was complemented by multidimensional homo- and heteronuclear high-resolution NMR spectroscopy of an extract of sheep brain tissue. The inclusion of spectra of model solutions in the program LC Model confirmed the assignments in situ. The first postmortem phase was characterized mainly by changes in the concentrations of metabolites usually observed in vivo and by the appearance of previously reported decay products. About 3 days postmortem, new metabolites, including free trimethylammonium, propionate, butyrate, and iso-butyrate, started to appear in situ. Since the observed metabolites and the time course is comparable in sheep and human brain tissue, the model system seems to be appropriate.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Pavillon 52 > Abt. Magnetresonanz-Spektroskopie und Methodologie, AMSM 04 Faculty of Medicine > Service Sector > Institute of Legal Medicine 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Unit Childrens Hospital > Forschungsgruppe Nephrologie / Hypertonie 04 Faculty of Medicine > Service Sector > Institute of Legal Medicine > Management 08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP) 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) 04 Faculty of Medicine > Department of Radiology, Neuroradiology and Nuclear Medicine (DRNN) > Institute of Diagnostic, Interventional and Paediatric Radiology > DCR Magnetic Resonance Spectroscopy and Methodology (AMSM)
UniBE Contributor:	Ith, Michael, Bigler, Peter, Scheurer, Eva, Kreis, Roland, Hofmann, Lucie, Dirnhofer, Richard, Boesch, Christoph Hans
Subjects:	600 Technology > 610 Medicine & health 500 Science > 570 Life sciences; biology 500 Science > 540 Chemistry
ISSN:	0740-3194
ISBN:	12418008
Publisher:	Wiley-Liss
Language:	English
Submitter:	Lucie Hofmann
Date Deposited:	04 Oct 2013 14:56
Last Modified:	02 Mar 2023 23:22
Publisher DOI:	10.1002/mrm.10294
PubMed ID:	12418008
Web of Science ID:	000179055200021
URI:	https://boris.unibe.ch/id/eprint/24145 (FactScience: 47116)