Characterisation of gp34, a GPI-anchored protein expressed by schizonts of Theileria parva and T. annulata

Xue, Gondga; von Schubert, Conrad; Hermann, Pascal; Peyer, Martina; Maushagen, Regina; Schmuckli-Maurer, Jacqueline; Bütikofer, Peter; Langsley, Gordon; Dobbelaere, Dirk, (2010). Characterisation of gp34, a GPI-anchored protein expressed by schizonts of Theileria parva and T. annulata. Molecular and biochemical parasitology, 172(2), pp. 113-20. Amsterdam: Elsevier 10.1016/j.molbiopara.2010.03.018

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Using bioinformatics tools, we searched the predicted Theileria annulata and T. parva proteomes for putative schizont surface proteins. This led to the identification of gp34, a GPI-anchored protein that is stage-specifically expressed by schizonts of both Theileria species and is downregulated upon induction of merogony. Transfection experiments in HeLa cells showed that the gp34 signal peptide and GPI anchor signal are also functional in higher eukaryotes. Epitope-tagged Tp-gp34, but not Ta-gp34, expressed in the cytosol of COS-7 cells was found to localise to the central spindle and midbody. Overexpression of Tp-gp34 and Ta-gp34 induced cytokinetic defects and resulted in accumulation of binucleated cells. These findings suggest that gp34 could contribute to important parasite-host interactions during host cell division.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Molecular Pathobiology
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine

UniBE Contributor:

Von Schubert, Conrad; Schmuckli, Jacqueline; Bütikofer, Peter and Dobbelaere, Dirk,

ISSN:

0166-6851

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:12

Last Modified:

10 Jul 2014 16:23

Publisher DOI:

10.1016/j.molbiopara.2010.03.018

PubMed ID:

20381541

Web of Science ID:

000278907700007

URI:

https://boris.unibe.ch/id/eprint/2416 (FactScience: 204907)

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